Product Info Summary
SKU: | PA2077 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin A10/ANXA10 Antibody
View all Annexin A10 Antibodies
SKU/Catalog Number
PA2077
BA3034 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin A10/ANXA10 Antibody catalog # PA2077. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin A10/ANXA10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2077)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin A10, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2077 is reactive to ANXA10 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
37278 MW
Background of Annexin A10
ANXA10 (Annexin A10) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. By FISH, Morgan et al. (1999) mapped the ANXA10 gene to chromosome 4q33. Morgan et al. (1999) suggested that the ablation or depletion of principal calcium-binding sites in annexins A9 and A10, respectively, may dispose them to function in distinct tissue environments where calcium sensitivity is not a major functional determinant.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2077 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: A549 Cell, A549 Cell,
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Annexin A10 antibody, PA2077, Western blotting
Lane 1: A549 Cell Lysate
Lane 2: A549 Cell Lysate
Click image to see more details
Anti-Annexin A10 antibody, PA2077, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For ANXA10 (Source: Uniprot.org, NCBI)
Gene Name
ANXA10
Full Name
Annexin A10
Weight
37278 MW
Superfamily
annexin family
Alternative Names
Annexin A10;Annexin-10;Annexin-14;ANXA10;ANX14; ANXA10 ANX14 annexin A10 annexin A10|annexin 14|annexin-10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANXA10, check out the ANXA10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANXA10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Annexin A10/ANXA10 Antibody (PA2077)
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No publications found for PA2077
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Customer Q&As
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7 Customer Q&As for Anti-Annexin A10/ANXA10 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-Annexin A10/ANXA10 antibody PA2077. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-11
Question
I am interested in to test anti-Annexin A10/ANXA10 antibody PA2077 on rat colon for research purposes, then I may be interested in using anti-Annexin A10/ANXA10 antibody PA2077 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The products we sell, including anti-Annexin A10/ANXA10 antibody PA2077, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-29
Question
Is this PA2077 anti-Annexin A10/ANXA10 antibody reactive to the isotypes of ANXA10?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The immunogen of PA2077 anti-Annexin A10/ANXA10 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin A10(308-324aa HYKKALLAICAGDAEDY), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-03
Question
Is there a BSA free version of anti-Annexin A10/ANXA10 antibody PA2077 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Annexin A10/ANXA10 antibody PA2077 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-Annexin A10/ANXA10 antibody PA2077 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The anti-Annexin A10/ANXA10 antibody (PA2077) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-17
Question
I see that the anti-Annexin A10/ANXA10 antibody PA2077 works with IHC, what is the protocol used to produce the result images on the product page?
G. Kulkarni
Verified customer
Asked: 2014-10-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-14
Question
I have a question about product PA2077, anti-Annexin A10/ANXA10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Mangal
Verified customer
Asked: 2014-09-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2077 anti-Annexin A10/ANXA10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-11