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Product Info Summary
| SKU: | A00953S1525 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Topo I alpha (S1525) TOP2A Antibody
SKU/Catalog Number
A00953S1525
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Topo I alpha (S1525) TOP2A Antibody catalog # A00953S1525. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Topo I alpha (S1525) TOP2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00953S1525)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Ser1525. AA range:1482-1531
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00953S1525 is reactive to TOP2A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
174385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00953S1525 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
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Western blot analysis of 293T 3T3 COLO using p-Topo IIα (S1525) antibody. antibody was diluted at 1:1000
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Immunohistochemistry analysis of paraffin-embedded human brain, using TOP2A (Phospho-Ser1525) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 5', using TOP2A (Phospho-Ser1525) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.org, NCBI)
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174385 MW
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2; TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Topo I alpha (S1525) TOP2A Antibody (A00953S1525)
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