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Product Info Summary
| SKU: | P00059-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00059-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody catalog # P00059-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00059-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AHO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00059-1 is reactive to SMAD3 in Human, Mouse
Reconstitution
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
48081 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00059-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human gastric adenocarcinoma tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-Smad3 (S423/S425) expression in A549 cell lysate treated with TGF-?1.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.
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Immunofluorescent analysis of A549 cells treated with TGFβ , using Phospho-Smad3 (S423 + S425) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMAD3 (Source: Uniprot.org, NCBI)
Gene Name
SMAD3
Full Name
Mothers against decapentaplegic homolog 3
Weight
48081 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3;SMAD3;MADH3; SMAD3 HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3 SMAD family member 3 mothers against decapentaplegic homolog 3|MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD3, check out the SMAD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody (P00059-1)
Hello CJ!
P00059-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, Song P, Yu Z, Liu C, Zhu Q, Zheng J. Int J Clin Exp Med. 2014 Sep 15;7(9):2645-50. Ecollection 2014. Dexamethasone Attenuates Bleomycin-Induced Lung Fibrosis In Mice Through Tgf-??, Smad3 And Jak-Stat P...
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody P00059-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody (P00059-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
My team were content with the WB result of your anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody. However we have seen positive staining in pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Based on literature, pancreas does express SMAD3. Generally SMAD3 expresses in cytoplasm. Regarding which tissues have SMAD3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Colon carcinoma, Pubmed ID: 9464505
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2019-09-19
Question
Do you have a similar anti-pSMAD3 antibodies from a different host species than rabbit for double immunostaining?
Verified customer
Asked: 2019-05-06
Answer
Unfortunately, we currently do not provide anti-phospho-SMAD3 antibodies from a different host species than rabbit.
Boster Scientific Support
Answered: 2019-05-06
Question
We ordered your anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody for IF on brain a few years ago. I am using human, and I plan to use the antibody for ICC next. We are interested in examining brain as well as erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
I looked at the website and datasheets of our anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody and I see that P00059-1 has been validated on human in both IF and ICC. Thus P00059-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-02
Question
We have seen staining in mouse cervix carcinoma. What should we do? Is anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
According to literature cervix carcinoma does express SMAD3. According to Uniprot.org, SMAD3 is expressed in amniotic fluid, placenta, colon carcinoma, brain, pancreas, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have SMAD3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648
Colon carcinoma, Pubmed ID: 9464505
Erythroleukemia, Pubmed ID: 23186163
Pancreas, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-08-30
Question
We need using your anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody for immune system development studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
We appreciate your inquiry. This P00059-1 anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal antibody is tested on a549 cells. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-02-02
