Product Info Summary
SKU: | P00018-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody
View all Sirtuin 1/SIRT1 Antibodies
SKU/Catalog Number
P00018-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody catalog # P00018-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00018-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FDA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-SIRT1 (S47)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00018-1 is reactive to SIRT1 in Human
Applications
P00018-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
185 kDa
Calculated molecular weight
81681 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
![p00018 1 wb p00018 1 wb](https://www.bosterbio.com/media/catalog/product/p/0/p00018-1-wb.jpg)
Click image to see more details
Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.
![p00018 1 ihc p00018 1 ihc](https://www.bosterbio.com/media/catalog/product/p/0/p00018-1-ihc.jpg)
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Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-SIRT1 (S47) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SIRT1 (Source: Uniprot.org, NCBI)
Gene Name
SIRT1
Full Name
NAD-dependent protein deacetylase sirtuin-1
Weight
81681 MW
Superfamily
sirtuin family
Alternative Names
EC 3.5.1; S. cerevisiae, homolog) 1; SIR2alpha; SIR2L1; SIR2-like protein 1; SIRT1; Sirtuin 1 SIRT1 SIR2, SIR2L1, SIR2alpha sirtuin 1 NAD-dependent protein deacetylase sirtuin-1|NAD-dependent protein deacylase sirtuin-1|SIR2-like protein 1|regulatory protein SIR2 homolog 1|sirtuin type 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT1, check out the SIRT1 Infographic
![SIRT1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody (P00018-1)
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No publications found for P00018-1
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody
Question
We bought anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody for WB on prostate in a previous project. I am using human, and We are going to use the antibody for IF next. I would like examining prostate as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I have checked the website and datasheets of our anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody and it seems that P00018-1 has been validated on human in both WB and IF. Thus P00018-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-26
Question
We are currently using anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody P00018-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody (P00018-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
My colleagues were well pleased with the WB result of your anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody. However we have seen positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Based on literature, liver does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-06-07
Question
We have seen staining in human right adrenal gland. Do you have any suggestions? Is anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody supposed to stain right adrenal gland positively?
R. Mangal
Verified customer
Asked: 2014-07-17
Answer
Based on literature right adrenal gland does express SIRT1. Based on Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2014-07-17