Product Info Summary
SKU: | PB9982 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ALDH3A2 Antibody Picoband®
SKU/Catalog Number
PB9982
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ALDH3A2 Antibody Picoband® catalog # PB9982. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH3A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9982)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9982 is reactive to ALDH3A2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
54848 MW
Background of FALDH
Fatty aldehyde dehydrogenase (or Long-chain-aldehyde dehydrogenase) is an aldehyde dehydrogenase enzyme that in human is encoded in the ALDH3A2 gene on chromosome 17. ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes into fatty acids. It is known to act on a variety of both saturated and unsaturated aliphatic aldehydes between 6 to 24 carbons in length, as well as dihydrophytal, a 20-carbon branched chain aldehyde. It requires NAD+ as a co-factor. The encoded enzyme is responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. ALD3H2 is expressed in the human liver and has been found to localize the microsome fraction inside the cell.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9982 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat liver tissue, rat testis tissue, human Hela whole cell, human A431 whole cell, human MCF-7 whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ALDH3A2 using anti-ALDH3A2 antibody (PB9982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A2 antigen affinity purified polyclonal antibody (Catalog # PB9982) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A2 at approximately 55 kDa. The expected band size for ALDH3A2 is at 55 kDa.
Click image to see more details
Figure 2. IHC analysis of ALDH3A2 using anti-ALDH3A2 antibody (PB9982).
ALDH3A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALDH3A2 Antibody (PB9982) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ALDH3A2 (Source: Uniprot.org, NCBI)
Gene Name
ALDH3A2
Full Name
Aldehyde dehydrogenase family 3 member A2
Weight
54848 MW
Superfamily
aldehyde dehydrogenase family
Alternative Names
Fatty aldehyde dehydrogenase;1.2.1.3;Aldehyde dehydrogenase 10;Aldehyde dehydrogenase family 3 member A2;Microsomal aldehyde dehydrogenase;ALDH3A2;ALDH10, FALDH; ALDH3A2 ALDH10, FALDH, SLS aldehyde dehydrogenase 3 family member A2 aldehyde dehydrogenase family 3 member A2|aldehyde dehydrogenase 10|fatty aldehyde dehydrogenase|microsomal aldehyde dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDH3A2, check out the ALDH3A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDH3A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALDH3A2 Antibody Picoband® (PB9982)
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Customer Q&As
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5 Customer Q&As for Anti-ALDH3A2 Antibody Picoband®
Question
Is this PB9982 anti-ALDH3A2 antibody reactive to the isotypes of ALDH3A2?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of PB9982 anti-ALDH3A2 antibody is E.coli-derived human ALDH3A2 recombinant protein (Position: M1-Q100). Human ALDH3A2 shares 78% amino acid (aa) sequence identity with both mouse and rat ALDH3A2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
Is there a BSA free version of anti-ALDH3A2 antibody PB9982 available?
E. Walker
Verified customer
Asked: 2020-01-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ALDH3A2 antibody PB9982 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-ALDH3A2 antibody PB9982 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The anti-ALDH3A2 antibody (PB9982) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis trachea using anti-ALDH3A2 antibody PB9982. Let me know if you need anything else.
B. Baker
Verified customer
Asked: 2019-03-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-22
Question
I was wanting to use your anti-ALDH3A2 antibody for WB for human testis trachea on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis trachea identification?
D. Williams
Verified customer
Asked: 2014-10-17
Answer
You can see on the product datasheet, PB9982 anti-ALDH3A2 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-17