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Product Info Summary
| SKU: | M00018 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SIRT1 Monoclonal Antibody
View all Sirtuin 1/SIRT1 Antibodies
SKU/Catalog Number
M00018
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SIRT1 Monoclonal Antibody catalog # M00018. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00018)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SIRT1 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00018 is reactive to SIRT1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00018 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human 293T whole cell, human K562 whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SIRT1 using anti-SIRT1 antibody (M00018).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified monoclonal antibody (Catalog # M00018) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using SIRT1 Antibody.
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Immunofluorescent analysis of Hela cells, using SIRT1 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SIRT1 (Source: Uniprot.org, NCBI)
Gene Name
SIRT1
Full Name
NAD-dependent protein deacetylase sirtuin-1
Weight
88068 MW
Superfamily
sirtuin family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; SIRT1 SIR2, SIR2L1, SIR2alpha sirtuin 1 NAD-dependent protein deacetylase sirtuin-1|NAD-dependent protein deacylase sirtuin-1|SIR2-like protein 1|regulatory protein SIR2 homolog 1|sirtuin type 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT1, check out the SIRT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT1 Monoclonal Antibody (M00018)
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No publications found for M00018
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Customer Q&As
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16 Customer Q&As for Anti-SIRT1 Monoclonal Antibody
Question
I am interested in using your anti-SIRT1 Monoclonal antibody for negative regulation of cellular senescence studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We appreciate your inquiry. This M00018 anti-SIRT1 Monoclonal antibody is tested on hela cells. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-30
Question
I would like to test anti-SIRT1 Monoclonal antibody M00018 on human leukemic t-cell for research purposes, then I may be interested in using anti-SIRT1 Monoclonal antibody M00018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The products we sell, including anti-SIRT1 Monoclonal antibody M00018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-19
Question
I see that the anti-SIRT1 Monoclonal antibody M00018 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-09
Question
We ordered your anti-SIRT1 Monoclonal antibody for IP on cervix carcinoma a few months ago. I am using human, and We want to use the antibody for WB next. We want examining cervix carcinoma as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Kulkarni
Verified customer
Asked: 2019-08-14
Answer
I looked at the website and datasheets of our anti-SIRT1 Monoclonal antibody and I see that M00018 has been validated on human in both IP and WB. Thus M00018 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-14
Question
Does M00018 anti-SIRT1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Miller
Verified customer
Asked: 2019-08-09
Answer
You can see on the product datasheet, M00018 anti-SIRT1 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-09
Question
Does anti-SIRT1 Monoclonal antibody M00018 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to the expression profile of leukemic t-cell, SIRT1 is highly expressed in leukemic t-cell. So, it is likely that anti-SIRT1 Monoclonal antibody M00018 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-09
Question
My question regarding product M00018, anti-SIRT1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Yang
Verified customer
Asked: 2019-08-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00018 anti-SIRT1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-08
Question
Is there a BSA free version of anti-SIRT1 Monoclonal antibody M00018 available?
G. Zhao
Verified customer
Asked: 2019-07-15
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SIRT1 Monoclonal antibody M00018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-15
Question
Is this M00018 anti-SIRT1 Monoclonal antibody reactive to the isotypes of SIRT1?
Verified Customer
Verified customer
Asked: 2018-08-29
Answer
The immunogen of M00018 anti-SIRT1 Monoclonal antibody is A synthesized peptide derived from human SIRT1 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-29
Question
I was wanting to use your anti-SIRT1 Monoclonal antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
As indicated on the product datasheet, M00018 anti-SIRT1 Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-SIRT1 Monoclonal antibody M00018. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-12
Question
We have seen staining in human cervix carcinoma. What should we do? Is anti-SIRT1 Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
According to literature cervix carcinoma does express SIRT1. According to Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2017-09-11
Question
Is a blocking peptide available for product anti-SIRT1 Monoclonal antibody (M00018)?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
We do provide the blocking peptide for product anti-SIRT1 Monoclonal antibody (M00018). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-11
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-SIRT1 Monoclonal antibody M00018. Please let me know if you require anything else.
T. Mangal
Verified customer
Asked: 2017-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-25
Question
My team were happy with the WB result of your anti-SIRT1 Monoclonal antibody. However we have seen positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?
V. Li
Verified customer
Asked: 2017-06-28
Answer
According to literature, cervix carcinoma erythroleukemia does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2017-06-28
Question
We are currently using anti-SIRT1 Monoclonal antibody M00018 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
G. Patel
Verified customer
Asked: 2017-02-15
Answer
The anti-SIRT1 Monoclonal antibody (M00018) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-15
