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Product Info Summary
| SKU: | A02538-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FNDC5 Antibody Picoband®
View all Irisin/FNDC5 Antibodies
SKU/Catalog Number
A02538-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FNDC5 Antibody Picoband® catalog # A02538-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FNDC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02538-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FNDC5 recombinant protein (Position: D32-E143).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02538-1 is reactive to FNDC5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23.659kDa
Background of Irisin/FNDC5
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02538-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
Protein Target Info & Infographic
Gene/Protein Information For FNDC5 (Source: Uniprot.org, NCBI)
Gene Name
FNDC5
Full Name
Fibronectin type III domain-containing protein 5
Weight
23.659kDa
Alternative Names
Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin; FNDC5; FRCP2 FNDC5 FRCP2, irisin fibronectin type III domain containing 5 fibronectin type III domain-containing protein 5|fibronectin type III repeat-containing protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FNDC5, check out the FNDC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FNDC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FNDC5 Antibody Picoband® (A02538-1)
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Customer Q&As
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3 Customer Q&As for Anti-FNDC5 Antibody Picoband®
Question
We are currently using anti-FNDC5 antibody A02538-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-FNDC5 antibody (A02538-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-FNDC5 antibody (A02538-1)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We do provide the blocking peptide for product anti-FNDC5 antibody (A02538-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-15
Question
See attached the WB image, lot number and protocol we used for quadriceps femoris using anti-FNDC5 antibody A02538-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
