Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody

Sirtuin 1/SIRT1 antibody

Boster Bio Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody catalog # P00018. Tested in WB application. This antibody reacts with Human.

Product Info Summary

SKU: P00018
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody

View all Sirtuin 1/SIRT1 Antibodies

SKU/Catalog Number

P00018

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody catalog # P00018. Tested in WB application. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00018)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HAI-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SIRT1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00018 is reactive to SIRT1 in Human

Applications

P00018 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

185 kDa

Calculated molecular weight

81681 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For SIRT1 (Source: Uniprot.org, NCBI)

Gene Name

SIRT1

Full Name

NAD-dependent protein deacetylase sirtuin-1

Weight

81681 MW

Superfamily

sirtuin family

Alternative Names

EC 3.5.1; S. cerevisiae, homolog) 1; SIR2alpha; SIR2L1; SIR2-like protein 1; SIRT1; Sirtuin 1 SIRT1 SIR2, SIR2L1, SIR2alpha sirtuin 1 NAD-dependent protein deacetylase sirtuin-1|NAD-dependent protein deacylase sirtuin-1|SIR2-like protein 1|regulatory protein SIR2 homolog 1|sirtuin type 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIRT1, check out the SIRT1 Infographic

SIRT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal Antibody

Question

We are currently using anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody P00018 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

J. Zhao

Verified customer

Asked: 2020-04-07

Answer

The anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody (P00018) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-07

Question

We are interested in using your anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody for protein deacetylation studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

We appreciate your inquiry. This P00018 anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody is validated on 293t cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

We have observed staining in human cervix carcinoma. What should we do? Is anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

According to literature cervix carcinoma does express SIRT1. According to Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378

Boster Scientific Support

Answered: 2019-08-12

Question

We were happy with the WB result of your anti-Phospho-SIRT1 (T530) Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell sirtt1 75 kda fragment: cytoplasm using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

Based on literature, leukemic t-cell does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378

Boster Scientific Support

Answered: 2019-06-28

Order DetailsPrice
P00018

100μL

$370
P00018-30ul

30μL sample

$99
P00018-carrier-free-1ml

Carrier Free

$2590

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