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Product Info Summary
| SKU: | A00818S176 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-RIP2 (S176) RIPK2 Antibody
SKU/Catalog Number
A00818S176
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-RIP2 (S176) RIPK2 Antibody catalog # A00818S176. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-RIP2 (S176) RIPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00818S176)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RIPK2 around the phosphorylation site of Ser176. AA range:146-195
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00818S176 is reactive to RIPK2 in Human, Mouse
Reconstitution
Calculated molecular weight
61195 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00818S176 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RIPK2 (Phospho-Ser176) antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using RIPK2 (Phospho-Ser176) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with UV 15', using RIPK2 (Phospho-Ser176) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For RIPK2 (Source: Uniprot.org, NCBI)
Gene Name
RIPK2
Full Name
Receptor-interacting serine/threonine-protein kinase 2
Weight
61195 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor-interacting serine/threonine-protein kinase 2;2.7.11.1;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;2.7.10.2;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092; RIPK2 CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 receptor interacting serine/threonine kinase 2 receptor-interacting serine/threonine-protein kinase 2|CARD-carrying kinase|CARD-containing IL-1 beta ICE-kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|RIP-2|growth-inhibiting gene 30|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase|receptor-interacting protein 2|tyrosine-protein kinase RIPK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK2, check out the RIPK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-RIP2 (S176) RIPK2 Antibody (A00818S176)
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