Click image to see more details
Product Info Summary
| SKU: | PB9010 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNF alpha Antibody Picoband®
SKU/Catalog Number
PB9010
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF alpha Antibody Picoband® catalog # PB9010. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9010)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9010 is reactive to Tnf in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
25806 MW
Background of TNF-alpha
TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9010 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: PC-12 Whole Cell, Rat Spleen Tissue, Rat Brain Tissue, Rat Kidney Tissue, Rat Liver Tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PB9010).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PC-12 Whole Cell Lysate,
Lane 2: Rat Spleen Tissue Lysate,
Lane 3: Rat Brain Tissue Lysate,
Lane 4: Rat Kidney Tissue Lysate,
Lane 5: Rat Liver Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PB9010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 26KD. The expected band size for TNF alpha is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For Tnf (Source: Uniprot.org, NCBI)
Gene Name
Tnf
Full Name
Tumor necrosis factor
Weight
25806 MW
Superfamily
Tumor necrosis factor family
Alternative Names
Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2; TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnf, check out the Tnf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF alpha Antibody Picoband® (PB9010)
Hello CJ!
PB9010 has been cited in 78 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li HN,Yang QQ,Wang WT,Tian X,Feng F,Zhang ST,Xia YT,Wang JX,Zou YW,Wang JY,Zeng XY.Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.J Neuroinflammation.2021 Jul 5;18(1):150.doi:10.1186/s12974-021-02198-9.PMID:34225736;PMCID:PMC8258957.
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION: 1:500
Wang B,Gao C,Zhang P,Sun W,Zhang J,Gao J.The increased motion of lumbar induces ligamentum flavum hypertrophy in a rat model. BMC Musculoskelet Disord.2021 Apr 6;22(1):334.doi:10.1186/s12891-021-04203-x.PMID:33823825.
Species: Human,Rat
PB9010 usage in article: APP:IHC, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:100
Yang Ping,Yingpeng Li,Shaowa Lü,Yali Sun,Wanmeng Zhang,Jialin Wu,Ting Liu,Yongji Li,A study of nanometre aggregates formation mechanism and antipyretic effect in Bai-Hu-Tang, an ancient Chinese herbal decoction,Biomedicine & Pharmacotherapy,Volume 124,202
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:HYPOTHALMIC TISSUE, DILUTION:NA
Yang QQ,Li HN,Zhang ST,Yu YL,Wei W,Zhang X,Wang JY,Zeng XY.Red nucleus IL-6 mediates the maintenance of neuropathic pain by inducing the productions of TNF-α and IL-1β through the JAK2/STAT3 and ERK signaling pathways.Neuropathology.2020 Aug;40(4):347-357
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:1:300
Guo Y,Gu R,Yu J,Lei B,Gan D,Xu G.Synthetic Glucocorticoid-Induced Leucine Zipper Peptide Inhibits Lipopolysaccharide-Induced Ocular Inflammation in Rats.Ophthalmic Res.2020;63(4):434-442.doi:10.1159/000505 003.Epub 2019 Nov 26.PMID:31770752.
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:1:500
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Zhao B,Li S,Guo Z,Chen Z,Zhang X,Xu C,Chen J,Wei C. Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways. Clin Exp Pharmacol Physiol.2020 Nov 11.doi:10.1111/1440-1681.13437.Ep
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HSCS, DILUTION:NA
Huang CL,Liu F,Zhang YY,Lin J,Fu M,Li YL, Zhou C,Li CJ,Shen JF. Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. J Neurophysiol.2020 Dec 16.doi:10.1152/jn.00646. 202
Species: Rat
PB9010 usage in article: APP:WB, SAMPLE:TG CELL AND SPVC CELL, DILUTION:1:1000
The effect of oral and intraurethral trimetazidine use on urethral healing
Synergistic action of inflammation and lipid dysmetabolism on kidney damage in rats
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TNF alpha Antibody Picoband®
Question
I am looking for using your anti-TNF alpha antibody for response to drug studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
We appreciate your inquiry. This PB9010 anti-TNF alpha antibody is tested on rat liver tissue, spleen tissue, tissue lysate, brain tissue, kidney tissue. It is guaranteed to work for WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-08
Question
I was wanting to use to test anti-TNF alpha antibody PB9010 on rat leukocyte for research purposes, then I may be interested in using anti-TNF alpha antibody PB9010 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The products we sell, including anti-TNF alpha antibody PB9010, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-24
Question
We have been able to see staining in rat prostatic carcinoma. Are there any suggestions? Is anti-TNF alpha antibody supposed to stain prostatic carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
According to literature prostatic carcinoma does express TNF. According to Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2019-10-14
Question
Does anti-TNF alpha antibody PB9010 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of leukocyte, TNF is highly expressed in leukocyte. So, it is likely that anti-TNF alpha antibody PB9010 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-09-18
Question
I was wanting to use your anti-TNF alpha antibody for WB for rat leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
It shows on the product datasheet, PB9010 anti-TNF alpha antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
Question
Our team were satisfied with the WB result of your anti-TNF alpha antibody. However we have seen positive staining in prostatic carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
From literature, prostatic carcinoma does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2019-01-24
Question
I see that the anti-TNF alpha antibody PB9010 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-25
Question
See attached the WB image, lot number and protocol we used for leukocyte using anti-TNF alpha antibody PB9010. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-14
Question
Is this PB9010 anti-TNF alpha antibody reactive to the isotypes of TNF?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
The immunogen of PB9010 anti-TNF alpha antibody is E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-10
Question
Is a blocking peptide available for product anti-TNF alpha antibody (PB9010)?
W. Zhang
Verified customer
Asked: 2017-12-22
Answer
We do provide the blocking peptide for product anti-TNF alpha antibody (PB9010). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-22
Question
Will PB9010 anti-TNF alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
As indicated on the product datasheet, PB9010 anti-TNF alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-25
Question
Can you help my question with product PB9010, anti-TNF alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Li
Verified customer
Asked: 2016-11-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9010 anti-TNF alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-11-11
Question
We are currently using anti-TNF alpha antibody PB9010 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on dog tissues as well?
C. Taylor
Verified customer
Asked: 2014-09-04
Answer
The anti-TNF alpha antibody (PB9010) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-04
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-TNF alpha antibody PB9010. Let me know if you need anything else.
N. Li
Verified customer
Asked: 2014-07-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-16
Question
Do you have a BSA free version of anti-TNF alpha antibody PB9010 available?
R. Mitchell
Verified customer
Asked: 2014-01-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TNF alpha antibody PB9010 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-01-10
