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Product Info Summary
| SKU: | P10723 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Pragmin (Tyr413) Prag1 Antibody
SKU/Catalog Number
P10723
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Pragmin (Tyr413) Prag1 Antibody (Catalog # P10723). Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Pragmin (Tyr413) Prag1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P10723)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human Pragmin around the phosphorylation site of Y413.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P10723 is reactive to PRAG1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
149624 MW
Background of PRAG1
Plus-end-directed motor enzyme that is required for completion of cytokinesis.
Scherer S.E., Nature 440:346-351(2006).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P10723 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal antibody
Protein Target Info & Infographic
Gene/Protein Information For PRAG1 (Source: Uniprot.org, NCBI)
Gene Name
PRAG1
Full Name
Inactive tyrosine-protein kinase PRAG1
Weight
149624 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase SgK223;2.7.10.2;Sugen kinase 223;SGK223; PRAG1 NACK, PEAK2, PRAGMIN, SGK223 PEAK1 related, kinase-activating pseudokinase 1 inactive tyrosine-protein kinase PRAG1|PEAK family member 2|Pragmin, RND2 effector protein|homolog of rat pragma of Rnd2|sugen kinase 223|tyrosine-protein kinase PRAG1|tyrosine-protein kinase SgK223
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRAG1, check out the PRAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Pragmin (Tyr413) Prag1 Antibody (P10723)
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No publications found for P10723
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Pragmin (Tyr413) Prag1 Antibody
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-Pragmin (Tyr413) Polyclonal antibody P10723. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
Is this P10723 anti-Phospho-Pragmin (Tyr413) Polyclonal antibody reactive to the isotypes of PRAG1?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The immunogen of P10723 anti-Phospho-Pragmin (Tyr413) Polyclonal antibody is Synthesized peptide derived from human Pragmin around the phosphorylation site of Y413. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-14
Question
We are currently using anti-Phospho-Pragmin (Tyr413) Polyclonal antibody P10723 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-Phospho-Pragmin (Tyr413) Polyclonal antibody (P10723) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
Will anti-Phospho-Pragmin (Tyr413) Polyclonal antibody P10723 work on feline WB with spleen?
C. Miller
Verified customer
Asked: 2013-10-25
Answer
Our lab technicians have not tested anti-Phospho-Pragmin (Tyr413) Polyclonal antibody P10723 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-Pragmin (Tyr413) Polyclonal antibody P10723 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline spleen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-25
