Key=store_id
Key=entity_id, value=55240
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EZ0404
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2019-05-09 05:30:19
Key=updated_at, value=2024-09-26 01:02:20
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
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Key=free_shipping, value=No
Key=template, value=ez-set
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1365
Key=yoast_content_score, value=60
Key=price, value=500.0000
Key=weight, value=1.0000
Key=meta_title, value=Human IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) EZ0404 | Boster Bio
Key=meta_description, value=Human IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) EZ0404 is a quantitative ELISA for measuring IL4 in Human ell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Order now!
Key=image, value=/e/z/ez0404.png
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Key=thumbnail, value=/e/z/ez0404.png
Key=url_key, value=human-il-4-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0404-boster
Key=url_path, value=human-il-4-elisa-kit-ez-set-trade-diy-antibody-pairs
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Key=image_label, value=Human IL-4 EZ Set ELISA Kit standard curve
Key=small_image_label, value=Human IL-4 EZ Set ELISA Kit standard curve
Key=thumbnail_label, value=Human IL-4 EZ Set ELISA Kit standard curve
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Key=size, value=For 5 plates, 96 wells each, reagents only, plates are not included
Key=protein_name, value=Interleukin-4
Key=gene_full_name, value=Interleukin-4
Key=uniprot_id, value=D4HNR6
Key=assay_range, value=15.6 pg/ml - 1,000 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=anticoagulant, value=heparin or EDTA
Key=tmb_incubation_time, value=15-25min
Key=normal_range_citations, value=24808641
Key=source_company, value=Boster
Key=molecular_weight, value=17492 MW
Key=product_category, value=EZ-Set ELISA Kits (Ab Pairs)
Key=swatch_image, value=/e/z/ez0404.png
Key=rating_value, value=100
Key=review_count, value=10
Key=mp_exclude_sitemap, value=Yes
Key=capture_antibody, value=Mouse anti- human IL4 monoclonal antibody
Key=detection_antibody, value=Biotinylated goat anti- human IL4 polyclonal antibody
Key=cj_related_products, value=Allergy:EK0650|EK2111|A00490-3|A00490Y525-1|PROTP43405^B Cell Activation:A06972
Key=description, value=Human IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=short_description, value=Human IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=background, value=Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Key=gene_name, value=IL4
Key=synonyms, value=Interleukin-4 ;IL-4 ;B-cell stimulatory factor 1 ;Lymphocyte stimulatory factor 1 ;IL4 ;hCG_24113 ;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0404-CA	Mouse anti- human IL4 monoclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0404-DA	Biotinylated goat anti- human IL4 polyclonal antibody (Detection Antibody)	500 µl, 10.3 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0404-ST	Lyophilized recombinant human IL4 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Key=subcellular_localization, value= Secreted .
Key=protein_function, value= Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. .
Key=sample_data, value=

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.019	0.030	0.062	0.127	0.229	0.539	0.980	1.851

Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: H25-S153
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=componentsneeded, value=

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered.
10. Color Developing Reagent: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T).
  PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered.
  PBS-T: 0.1% Tween? 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Key=preparation_step_2, value=1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10 ng of lyophilized human IL4 standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10 ng/ml using 1 ml of Reagent Diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2)Dilution of Human IL4 Standard
• Number tubes 1 - 8. Final Concentrations to be Tube # 1 - 1,000 pg/ml, # 2 - 500 pg/ml, # 3 - 250 pg/ml, # 4 - 125 pg/ml, # 5 - 62.5 pg/ml, # 6 - 31.25 pg/ml, # 7 - 15.625 pg/ml, # 8 - 0.0 (Blank).
• To generate standard # 1, add 100 µl of the reconstituted standard stock solution of 10 ng/ml and 900 µl of Reagent Diluent to tube # 1 for a final volume of 1,000 µl. Mix thoroughly.
• Add 300 µl of Reagent Diluent to tubes # 2 - 7.
• To generate standard # 2, add 300 µl of standard # 1 from tube # 1 to tube # 2 for a final volume of 600 µl. Mix thoroughly.
• To generate standard # 3, add 300 µl of standard # 2 from tube # 2 to tube # 3 for a final volume of 600 µl. Mix thoroughly.
• Continue the serial dilution for tube # 4 - 7.
• Tube # 8 is a blank standard to be used with every experiment.
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