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Product Info Summary
| SKU: | A32113Y413 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-Pragmin (Y413) PRAG1 Antibody
SKU/Catalog Number
A32113Y413
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Pragmin (Y413) PRAG1 Antibody catalog # A32113Y413. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Pragmin (Y413) PRAG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32113Y413)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Pragmin (phospho Tyr413)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32113Y413 is reactive to PRAG1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
149624 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32113Y413 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) polyclonal antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Pragmin (Phospho-Tyr413) antibody
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Western blot analysis of lysate from A549 cells, using phospho-Pragmin (Phospho-Tyr413) antibody.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1, primary antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200
Protein Target Info & Infographic
Gene/Protein Information For PRAG1 (Source: Uniprot.org, NCBI)
Gene Name
PRAG1
Full Name
Inactive tyrosine-protein kinase PRAG1
Weight
149624 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase PRAG1 PRAG1 NACK, PEAK2, PRAGMIN, SGK223 PEAK1 related, kinase-activating pseudokinase 1 inactive tyrosine-protein kinase PRAG1|PEAK family member 2|Pragmin, RND2 effector protein|homolog of rat pragma of Rnd2|sugen kinase 223|tyrosine-protein kinase PRAG1|tyrosine-protein kinase SgK223
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRAG1, check out the PRAG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Pragmin (Y413) PRAG1 Antibody (A32113Y413)
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