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Product Info Summary
| SKU: | A08127 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cdc42 effector protein 3 CDC42EP3 Antibody
SKU/Catalog Number
A08127
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cdc42 effector protein 3 CDC42EP3 Antibody (Catalog # A08127). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc42 effector protein 3 CDC42EP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08127)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CDC42EP3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08127 is reactive to CDC42EP3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
27678 MW
Background of CDC42EP3
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts.
Joberty G., Mol. Cell. Biol. 19:6585-6597(1999).
Hirsch D.S., J. Biol. Chem. 276:875-883(2001).
Wiemann S., Genome Res. 11:422-435(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08127 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using CDC42EP3 antibody A08127.
Protein Target Info & Infographic
Gene/Protein Information For CDC42EP3 (Source: Uniprot.org, NCBI)
Gene Name
CDC42EP3
Full Name
Cdc42 effector protein 3
Weight
27678 MW
Superfamily
BORG/CEP family
Alternative Names
Cdc42 effector protein 3;Binder of Rho GTPases 2;MSE55-related Cdc42-binding protein;CDC42EP3;BORG2, CEP3; CDC42EP3 BORG2, CEP3, UB1 CDC42 effector protein 3 cdc42 effector protein 3|CDC42 effector protein (Rho GTPase binding) 3|CRIB-containing BORG2 protein|MSE55-related Cdc42-binding protein|MSE55-related protein|binder of Rho GTPases 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42EP3, check out the CDC42EP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42EP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc42 effector protein 3 CDC42EP3 Antibody (A08127)
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No publications found for A08127
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Customer Q&As
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4 Customer Q&As for Anti-Cdc42 effector protein 3 CDC42EP3 Antibody
Question
Is there a BSA free version of anti-CDC42EP3 antibody A08127 available?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDC42EP3 antibody A08127 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-19
Question
I was wanting to use your anti-CDC42EP3 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
You can see on the product datasheet, A08127 anti-CDC42EP3 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-10
Question
We are currently using anti-CDC42EP3 antibody A08127 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
B. Krishna
Verified customer
Asked: 2018-02-07
Answer
The anti-CDC42EP3 antibody (A08127) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-07
Question
Please see the WB image, lot number and protocol we used for testis using anti-CDC42EP3 antibody A08127. Please let me know if you require anything else.
M. Singh
Verified customer
Asked: 2017-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-03
