Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody

Product Name

Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody

View all RNA Polymerase II/POLR2A Antibodies

SKU/Catalog Number

P01029-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody catalog # P01029-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01029-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABCE-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human POLR2A

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P01029-2 is reactive to POLR2A in Human, Mouse, Rat

Applications

P01029-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

217176 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:40

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate treated with Lambda Phosphatase; (2) HeLa cell lysate.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human spleen, using Phospho-POLR2A (S5) Antibody.

Click image to see more details

Immunofluorescent analysis of Hela cells, using Phospho-POLR2A (S5) Antibody .

Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.48; EC 2.7.7.6; hRPB220; hsRPB1; MGC75453; POLR2; POLR2A; POLRA; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RNA Pol 2; RNA Polymerase 2; RNA polymerase II subunit B1; RNA Polymerase II; RNA-directed RNA polymerase II subunit RPB1; RNAPII; RPB1; RPBh1; RpIILS; RPO2; RPOL2 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A

For more info on POLR2A, check out the POLR2A Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the POLR2A Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis