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Product Info Summary
| SKU: | A01029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-RNA polymerase II RPB1/POLR2A Antibody
View all RNA Polymerase II/POLR2A Antibodies
SKU/Catalog Number
A01029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RNA polymerase II RPB1/POLR2A Antibody catalog # A01029. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNA polymerase II RPB1/POLR2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01029)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human POLR2A recombinant protein (Position: A1148-H1384).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01029 is reactive to POLR2A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
217.176kDa
Background of RNA Polymerase II/POLR2A
DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01029 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
IHC: human mammary cancer tissue, mouse testis tissue, rat small intestine tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
POLR2A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
POLR2A was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
POLR2A was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
POLR2A was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)
Gene Name
POLR2A
Full Name
DNA-directed RNA polymerase II subunit RPB1
Weight
217.176kDa
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2 POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2A, check out the POLR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNA polymerase II RPB1/POLR2A Antibody (A01029)
Hello CJ!
A01029 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang H,Liu J,Yao F,Li X,Wang Y,Shao Y,Wang X,Kong J,Zhang X,Jiang T,Hou L.The integrator complex subunit 11 is involved in the post-diapaused embryonic development and stress response of Artemia sinica.Gene.2020 May 30;741:144548.doi:10.1016/j.gene.2020.144548.Epub 2020 Mar 9.PMID:32165292.
Species: Artemia sinica
A01029 usage in article: APP:WB, SAMPLE:LARVAE, DILUTION:NA
Heparanase mediates vascular endothelial growth factor gene transcription in high-glucose human retinal microvascular endothelial cells.
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Customer Q&As
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1 Customer Q&As for Anti-RNA polymerase II RPB1/POLR2A Antibody
Question
We are currently using anti-RNA polymerase II RPB1/POLR2A antibody A01029 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
The anti-RNA polymerase II RPB1/POLR2A antibody (A01029) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-01
