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Product Info Summary
| SKU: | P01029-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody
View all RNA Polymerase II/POLR2A Antibodies
SKU/Catalog Number
P01029-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody catalog # P01029-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01029-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human POLR2A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01029-1 is reactive to POLR2A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
80 kDa
Calculated molecular weight
217176 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01029-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: MCF7 cell
IHC: human spleen tissue
ICC/IF: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-POLR2A (S2) expression in (1) MCF7 cell lysate treated with Lambda Phosphatase; (2) MCF7 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human spleen, using Phospho-POLR2A (S2) Antibody.
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Immunofluorescent analysis of MCF7 cells, using Phospho-POLR2A (S2) Antibody .
Protein Target Info & Infographic
Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)
Gene Name
POLR2A
Full Name
DNA-directed RNA polymerase II subunit RPB1
Weight
217176 MW
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;2.7.7.6;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1;2.7.7.48;POLR2A;POLR2; POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2A, check out the POLR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody (P01029-1)
Hello CJ!
P01029-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Heparanase mediates vascular endothelial growth factor gene transcription in high-glucose human retinal microvascular endothelial cells.
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