Click image to see more details
Product Info Summary
| SKU: | P01293 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-PKCth (Ser676) PRKCQ Antibody
SKU/Catalog Number
P01293
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-PKCth (Ser676) PRKCQ Antibody (Catalog # P01293). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PKCth (Ser676) PRKCQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01293)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKCth.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01293 is reactive to PRKCQ in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
81865 MW
Background of PKC theta
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01293 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCθ (Phospho-Ser676) antibody P01293.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) antibody P01293(left) or the same antibody preincubated with blocking peptide(right).
Click image to see more details
Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) antibody P01293.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) antibody P01293.
Protein Target Info & Infographic
Gene/Protein Information For PRKCQ (Source: Uniprot.org, NCBI)
Gene Name
PRKCQ
Full Name
Protein kinase C theta type
Weight
81865 MW
Superfamily
protein kinase superfamily
Alternative Names
Protein kinase C theta type;2.7.11.13;nPKC-theta;PRKCQ;PRKCT; PRKCQ PRKCT, nPKC-theta protein kinase C theta protein kinase C theta type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCQ, check out the PRKCQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PKCth (Ser676) PRKCQ Antibody (P01293)
Hello CJ!
No publications found for P01293
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-PKCth (Ser676) PRKCQ Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-PKCth (Ser676) PRKCQ Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Phospho-PKCth (Ser676) PRKCQ Antibody
Question
Is a blocking peptide available for product anti-Phospho-PKCth (Ser676) antibody (P01293)?
C. Jones
Verified customer
Asked: 2020-02-21
Answer
We do provide the blocking peptide for product anti-Phospho-PKCth (Ser676) antibody (P01293). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-Phospho-PKCth (Ser676) antibody P01293 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Phospho-PKCth (Ser676) antibody (P01293) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Would anti-Phospho-PKCth (Ser676) antibody P01293 work on feline IHC with brain?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Our lab technicians have not validated anti-Phospho-PKCth (Ser676) antibody P01293 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-PKCth (Ser676) antibody P01293 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
Would P01293 anti-Phospho-PKCth (Ser676) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can see on the product datasheet, P01293 anti-Phospho-PKCth (Ser676) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-10
