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Product Info Summary
| SKU: | M00963 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IL22 Antibody Picoband® (monoclonal, 7F2)
SKU/Catalog Number
M00963
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL22 Antibody Picoband® (monoclonal, 7F2) catalog # M00963. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL22 Antibody Picoband® (monoclonal, 7F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00963)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
7F2
Isotype
Mouse IgG1
Immunogen
E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00963 is reactive to IL22 in Human
Reconstitution
Observed Molecular Weight
24 kDa
Calculated molecular weight
20.011kDa
Background of IL-22
Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00963 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human U-87MG whole cell, human A375 whole cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of HL-60 cells using anti-IL22 antibody (M00963).
Overlay histogram showing HL-60 cells stained with M00963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IL22 Antibody (M00963,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 2. Western blot analysis of IL22 using anti-IL22 antibody (M00963).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human A375 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IL22 antigen affinity purified monoclonal antibody (Catalog # M00963) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24KD. The expected band size for IL22 is at 20KD.
Protein Target Info & Infographic
Gene/Protein Information For IL22 (Source: Uniprot.org, NCBI)
Gene Name
IL22
Full Name
Interleukin-22
Weight
20.011kDa
Superfamily
IL-10 family
Alternative Names
Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL22; ILTIF, ZCYTO18; UNQ3099/PRO10096 IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL22, check out the IL22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL22 Antibody Picoband® (monoclonal, 7F2) (M00963)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-IL22 Antibody Picoband® (monoclonal, 7F2)
Question
See below the WB image, lot number and protocol we used for zone of skin using anti-IL22 antibody (monoclonal, 7F2) M00963. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-06
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-IL22 antibody (monoclonal, 7F2) supposed to stain blood positively?
R. Li
Verified customer
Asked: 2019-09-27
Answer
Based on literature blood does express IL22. Based on Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-27
Question
We purchased anti-IL22 antibody (monoclonal, 7F2) for WB on zone of skin in the past. I am using human, and We are going to use the antibody for Flow Cytometry next. I was wanting to use examining zone of skin as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
I have checked the website and datasheets of our anti-IL22 antibody (monoclonal, 7F2) and it appears that M00963 has been tested on human in both WB and Flow Cytometry. Thus M00963 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-26
Question
I would like to test anti-IL22 antibody (monoclonal, 7F2) M00963 on human zone of skin for research purposes, then I may be interested in using anti-IL22 antibody (monoclonal, 7F2) M00963 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-IL22 antibody (monoclonal, 7F2) M00963, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-IL22 antibody (monoclonal, 7F2) M00963 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-20
Question
Does M00963 anti-IL22 antibody (monoclonal, 7F2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Huang
Verified customer
Asked: 2019-02-07
Answer
You can see on the product datasheet, M00963 anti-IL22 antibody (monoclonal, 7F2) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-07
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for zone of skin using anti-IL22 antibody (monoclonal, 7F2) M00963. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-28
Question
Will anti-IL22 antibody (monoclonal, 7F2) M00963 work for WB with zone of skin?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
According to the expression profile of zone of skin, IL22 is highly expressed in zone of skin. So, it is likely that anti-IL22 antibody (monoclonal, 7F2) M00963 will work for WB with zone of skin.
Boster Scientific Support
Answered: 2018-12-19
Question
Do you have a BSA free version of anti-IL22 antibody (monoclonal, 7F2) M00963 available?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL22 antibody (monoclonal, 7F2) M00963 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-09
Question
My question regarding product M00963, anti-IL22 antibody (monoclonal, 7F2). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00963 anti-IL22 antibody (monoclonal, 7F2), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-20
Question
I was wanting to use your anti-IL22 antibody (monoclonal, 7F2) for WB for human zone of skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human zone of skin identification?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
It shows on the product datasheet, M00963 anti-IL22 antibody (monoclonal, 7F2) has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human zone of skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-27
Question
I was wanting to use using your anti-IL22 antibody (monoclonal, 7F2) for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on human a375 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
We appreciate your inquiry. This M00963 anti-IL22 antibody (monoclonal, 7F2) is validated on human a375 whole cell lysates. It is guaranteed to work for Flow Cytometry, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-15
Question
We were satisfied with the WB result of your anti-IL22 antibody (monoclonal, 7F2). However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?
V. Collins
Verified customer
Asked: 2018-01-24
Answer
Based on literature, blood does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-24
Question
Is this M00963 anti-IL22 antibody (monoclonal, 7F2) reactive to the isotypes of IL22?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
The immunogen of M00963 anti-IL22 antibody (monoclonal, 7F2) is E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-22
Question
We are currently using anti-IL22 antibody (monoclonal, 7F2) M00963 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
C. Krishna
Verified customer
Asked: 2017-11-02
Answer
The anti-IL22 antibody (monoclonal, 7F2) (M00963) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-02
Question
Is a blocking peptide available for product anti-IL22 antibody (monoclonal, 7F2) (M00963)?
R. Johnson
Verified customer
Asked: 2016-02-08
Answer
We do provide the blocking peptide for product anti-IL22 antibody (monoclonal, 7F2) (M00963). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-08
