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Product Info Summary
| SKU: | P01992 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody
SKU/Catalog Number
P01992
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody catalog # P01992. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01992)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. AA range:947-996
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01992 is reactive to EIF2AK3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
125216 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01992 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC-P 1:100-1:300
IF 1:50-200
ELISA 1:40000
Validation Images & Assay Conditions
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Immunofluorescence analysis of Hela cell. 1, PERK (Phospho- Thr981) polyclonal antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
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Western blot analysis of lysates from 1) 453, 2) AD293 , 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody BB133 J289 was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal antibody (5B7) J109 D656 D462 J435 F434 V416 D369 BB133 J109 L10 antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
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Immunofluorescence analysis of rat-heart tissue. 1, PERK (Phospho- Thr981) polyclonal antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, PERK (Phospho- Thr981) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, PERK (Phospho- Thr981) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, PERK (Phospho- Thr981) polyclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PEK/PERK (Phospho-Thr981) antibody
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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using PEK/PERK (Phospho-Thr981) antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK3 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK3
Full Name
Eukaryotic translation initiation factor 2-alpha kinase 3
Weight
125216 MW
Superfamily
protein kinase superfamily
Alternative Names
Eukaryotic translation initiation factor 2-alpha kinase 3;2.7.11.1;PRKR-like endoplasmic reticulum kinase;Pancreatic eIF2-alpha kinase;HsPEK;EIF2AK3;PEK, PERK; EIF2AK3 PEK, PERK, WRS eukaryotic translation initiation factor 2 alpha kinase 3 eukaryotic translation initiation factor 2-alpha kinase 3|PRKR-like endoplasmic reticulum kinase|pancreatic EIF2-alpha kinase|truncated eukaryotic translation initiation factor 2 alpha kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK3, check out the EIF2AK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody (P01992)
Hello CJ!
P01992 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Positive‐Feedback Regulation of Subchondral H‐Type Vessel Formation by Chondrocyte Promotes Osteoarthritis Development in Mice
Pei-pei Fang,Chen-wei Pan,Wei Lin,Jie Li,Shan-shan Huang,Guang-yao Zhou,Wen-jun Du,Qiang Li, "ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes",Mediators of Inflammation,vol.2020,Art
Species: Human
P01992 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1500
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-Phospho-PERK (T981) Rabbit Polyclonal Antibody
Question
Do you require any kind of licensing agreement with the researcher who wants to evaluate a Boster polyclonal antibody for possible inclusion in a commercialize product?
Verified customer
Asked: 2022-07-05
Answer
Yes, we need to have a licensing agreement, for many purposes but primarily to ensure the continuous supply of this product, which the researcher would need. We should ask them to discuss more details including the expected demand of this antibody. Since this is a polyclonal antibody we need to produce ahead of time and validate to ensure it keeps working properly for the customer.
Boster Scientific Support
Answered: 2022-07-05
Question
Can you confirm if P01992 is suitable for Western Blot?
Verified customer
Asked: 2020-01-10
Answer
The Anti-Phospho-PERK (T981) Antibody (P01992) is suitable for WB.
Boster Scientific Support
Answered: 2020-01-13
Question
See below the WB image, lot number and protocol we used for pancreas testis using anti-Phospho-PERK (T981) antibody P01992. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-Phospho-PERK (T981) antibody P01992 work for ELISA with pancreas testis?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
According to the expression profile of pancreas testis, EIF2AK3 is highly expressed in pancreas testis. So, it is likely that anti-Phospho-PERK (T981) antibody P01992 will work for ELISA with pancreas testis.
Boster Scientific Support
Answered: 2020-01-07
Question
I see that the anti-Phospho-PERK (T981) antibody P01992 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-02
Question
We have been able to see staining in rat pancreas testis. Are there any suggestions? Is anti-Phospho-PERK (T981) antibody supposed to stain pancreas testis positively?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to literature pancreas testis does express EIF2AK3. According to Uniprot.org, EIF2AK3 is expressed in nasal cavity mucosa, liver, pancreas testis, brain pancreas, brain, cervix carcinoma, among other tissues. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2019-05-21
Question
Will P01992 anti-Phospho-PERK (T981) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
You can see on the product datasheet, P01992 anti-Phospho-PERK (T981) antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-22
Question
My team were content with the WB result of your anti-Phospho-PERK (T981) antibody. However we have observed positive staining in cervix carcinoma endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is EIF2AK3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
From literature, cervix carcinoma does express EIF2AK3. Generally EIF2AK3 expresses in endoplasmic reticulum membrane. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2018-12-21
Question
Will anti-Phospho-PERK (T981) antibody P01992 work on canine IHC with nasal cavity mucosa?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
Our lab technicians have not tested anti-Phospho-PERK (T981) antibody P01992 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Phospho-PERK (T981) antibody P01992 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine nasal cavity mucosa in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-14
Question
I was wanting to use your anti-Phospho-PERK (T981) antibody for ELISA for mouse pancreas testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pancreas testis identification?
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
As indicated on the product datasheet, P01992 anti-Phospho-PERK (T981) antibody has been tested for ELISA, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse pancreas testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-31
Question
Is this P01992 anti-Phospho-PERK (T981) antibody reactive to the isotypes of EIF2AK3?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
The immunogen of P01992 anti-Phospho-PERK (T981) antibody is Synthesized peptide derived from human PERK around the phosphorylation site of T981. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-25
Question
Our lab want to know about to test anti-Phospho-PERK (T981) antibody P01992 on mouse pancreas testis for research purposes, then I may be interested in using anti-Phospho-PERK (T981) antibody P01992 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-Phospho-PERK (T981) antibody P01992, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
I have a question about product P01992, anti-Phospho-PERK (T981) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Krishna
Verified customer
Asked: 2017-06-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P01992 anti-Phospho-PERK (T981) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-21
Question
Our lab used your anti-Phospho-PERK (T981) antibody for ELISA on cervix carcinoma last year. I am using mouse, and We are going to use the antibody for IHC next. My question regards examining cervix carcinoma as well as nasal cavity mucosa in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
M. Li
Verified customer
Asked: 2015-09-17
Answer
I have checked the website and datasheets of our anti-Phospho-PERK (T981) antibody and I see that P01992 has been tested on mouse in both ELISA and IHC. Thus P01992 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-09-17
Question
We are currently using anti-Phospho-PERK (T981) antibody P01992 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
G. Edwards
Verified customer
Asked: 2015-07-20
Answer
The anti-Phospho-PERK (T981) antibody (P01992) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-20
Question
Do you have a BSA free version of anti-Phospho-PERK (T981) antibody P01992 available?
N. Williams
Verified customer
Asked: 2015-03-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-PERK (T981) antibody P01992 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-03-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas testis using anti-Phospho-PERK (T981) antibody P01992. Let me know if you need anything else.
C. Jackson
Verified customer
Asked: 2013-11-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-27
Question
Is a blocking peptide available for product anti-Phospho-PERK (T981) antibody (P01992)?
T. Miller
Verified customer
Asked: 2013-05-10
Answer
We do provide the blocking peptide for product anti-Phospho-PERK (T981) antibody (P01992). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-10
