Anti-Fibronectin FN1 Antibody Biotin Conjugated

Product Name

Anti-Fibronectin FN1 Antibody Biotin Conjugated

View all Fibronectin Antibodies

SKU/Catalog Number

B00564

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-Fibronectin FN1 Antibody Biotin Conjugated (Catalog # B00564). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Monkey.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Fibronectin FN1 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00564)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B00564 is reactive to FN1 in Bovine, Human, Monkey, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

42 kDa

Calculated molecular weight

262625 MW

Background of Fibronectin

Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

B00564 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
Fluorochrome Protein Value: 10-20
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For FN1 (Source: Uniprot.org, NCBI)

Alternative Names

Fibronectin biotin conjugation, Fibronectin, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3 FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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