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Product Info Summary
| SKU: | A01992 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-PERK EIF2AK3 Antibody
SKU/Catalog Number
A01992
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PERK EIF2AK3 Antibody (Catalog # A01992). Tested in IHC, IP, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PERK EIF2AK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01992)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01992 is reactive to EIF2AK3 in Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
125216 MW
Background of PERK
The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2 results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01992 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:20,000
IHC: 1:1,000
IF Microscopy: User optimized
IP: 10-30ul
WB: 1:500 – 1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PERK expression in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15ul anti-PERK. PERK was detected using rabbit anti-PERK Antiserum, not purified polyclonal antibody (Catalog # A01992. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2 , 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Click image to see more details
PERK was detected in paraffin-embedded sections of mouse mammary gland tissues using rabbit anti-PERK Antiserum, not purified polyclonal antibody (Catalog # A01992) at 1:1000. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK3 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK3
Full Name
Eukaryotic translation initiation factor 2-alpha kinase 3
Weight
125216 MW
Superfamily
protein kinase superfamily
Alternative Names
PKR-like Endoplasmic Reticulum Kinase, PERK, PKR-like endoplasmic reticulum kinase, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3 EIF2AK3 PEK, PERK, WRS eukaryotic translation initiation factor 2 alpha kinase 3 eukaryotic translation initiation factor 2-alpha kinase 3|PRKR-like endoplasmic reticulum kinase|pancreatic EIF2-alpha kinase|truncated eukaryotic translation initiation factor 2 alpha kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK3, check out the EIF2AK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PERK EIF2AK3 Antibody (A01992)
Hello CJ!
A01992 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
BCAT1 binds the RNA-binding protein ZNF423 to activate autophagy via the IRE1-XBP-1-RIDD axis in hypoxic PASMCs
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PERK EIF2AK3 Antibody
Question
We were happy with the WB result of your anti-PERK antibody. However we have observed positive staining in cervix carcinoma endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is EIF2AK3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
From what I have seen in literature, cervix carcinoma does express EIF2AK3. Generally EIF2AK3 expresses in endoplasmic reticulum membrane. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2020-04-24
Question
I was wanting to use your anti-PERK antibody for IP for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can see on the product datasheet, A01992 anti-PERK antibody has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
Does anti-PERK antibody A01992 work for IP with liver?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
According to the expression profile of liver, EIF2AK3 is highly expressed in liver. So, it is likely that anti-PERK antibody A01992 will work for IP with liver.
Boster Scientific Support
Answered: 2020-02-25
Question
Is there a BSA free version of anti-PERK antibody A01992 available?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PERK antibody A01992 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PERK antibody A01992. Let me know if you need anything else.
R. Kulkarni
Verified customer
Asked: 2019-09-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
My question regarding product A01992, anti-PERK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01992 anti-PERK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
We are currently using anti-PERK antibody A01992 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-PERK antibody (A01992) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
I am looking for using your anti-PERK antibody for cellular response to cold studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your inquiry. This A01992 anti-PERK antibody is tested on 293t cell lysate. It is guaranteed to work for IP, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-25
Question
We have tried in the past anti-PERK antibody for IHC on brain pancreas last year. I am using human, and We want to use the antibody for IP next. We are interested in examining brain pancreas as well as nasal cavity mucosa in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
I looked at the website and datasheets of our anti-PERK antibody and it seems that A01992 has been tested on human in both IHC and IP. Thus A01992 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-24
Question
Will A01992 anti-PERK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It shows on the product datasheet, A01992 anti-PERK antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
Is a blocking peptide available for product anti-PERK antibody (A01992)?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do provide the blocking peptide for product anti-PERK antibody (A01992). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-27
Question
Is this A01992 anti-PERK antibody reactive to the isotypes of EIF2AK3?
L. Wu
Verified customer
Asked: 2018-10-08
Answer
The immunogen of A01992 anti-PERK antibody is This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK (see link below for the full length sequence of the mouse gene product). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-08
Question
We have seen staining in human cervix carcinoma. Any tips? Is anti-PERK antibody supposed to stain cervix carcinoma positively?
N. Jones
Verified customer
Asked: 2017-06-27
Answer
From literature cervix carcinoma does express EIF2AK3. From Uniprot.org, EIF2AK3 is expressed in nasal cavity mucosa, liver, pancreas testis, brain pancreas, brain, cervix carcinoma, among other tissues. Regarding which tissues have EIF2AK3 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, and Pancreas, Pubmed ID: 10677345
Cervix carcinoma, Pubmed ID: 20068231
Liver, Pancreas, and Testis, Pubmed ID: 10026192
Boster Scientific Support
Answered: 2017-06-27
Question
I was wanting to use to test anti-PERK antibody A01992 on human liver for research purposes, then I may be interested in using anti-PERK antibody A01992 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Anderson
Verified customer
Asked: 2016-11-16
Answer
The products we sell, including anti-PERK antibody A01992, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-16
Question
I see that the anti-PERK antibody A01992 works with IP, what is the protocol used to produce the result images on the product page?
A. Jones
Verified customer
Asked: 2016-06-16
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-06-16
Question
I have attached the WB image, lot number and protocol we used for liver using anti-PERK antibody A01992. Please let me know if you require anything else.
K. Mangal
Verified customer
Asked: 2015-10-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-02
