Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1)

AMPK beta 2/PRKAB2 antibody

Boster Bio Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) catalog # M05077. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M05077
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1)

View all AMPK beta 2/PRKAB2 Antibodies

SKU/Catalog Number

M05077

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) catalog # M05077. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05077)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

6G1

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M05077 is reactive to PRKAB2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

34 kDa

Calculated molecular weight

30.302kDa

Background of AMPK beta 2/PRKAB2

5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05077 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells

Positive Control

WB: human Hela whole cell, human placenta tissue, human 293T whole cell, human A549 whole cell, human A375 whole cell, human A431 whole cell, human U20S whole cell, human K562 whole cell
FCM: PC-3 cell

Validation Images & Assay Conditions

Gene/Protein Information For PRKAB2 (Source: Uniprot.org, NCBI)

Gene Name

PRKAB2

Full Name

5'-AMP-activated protein kinase subunit beta-2

Weight

30.302kDa

Superfamily

5'-AMP-activated protein kinase beta subunit family

Alternative Names

5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2; PRKAB2 PRKAB2 protein kinase AMP-activated non-catalytic subunit beta 2 5-AMP-activated protein kinase subunit beta-2|5-AMP-activated protein kinase, beta-2 subunit|AMP-activated protein kinase beta 2 non-catalytic subunit|AMPK beta 2|AMPK beta-2 chain|AMPK subunit beta-2|protein kinase, AMP-activated, beta 2 non-catalytic subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKAB2, check out the PRKAB2 Infographic

PRKAB2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M05077

100μg

$370
M05077-10ug

10μg sample (liquid)

$99
M05077-Biotin

100 μg Biotin conjugated

$570
M05077-Cy3

100 μg Cy3 conjugated

$570
M05077-Dylight488

100 μg Dylight488 conjugated

$570
M05077-Dylight550

100 μg Dylight550 conjugated

$570
M05077-Dylight594

100 μg Dylight594 conjugated

$570
M05077-FITC

100 μg FITC conjugated

$570
M05077-HRP

100 μg HRP conjugated

$570
M05077-APC

100 μg APC conjugated

$670
M05077-PE

100 μg PE conjugated

$670
M05077-iFluor647

100 μg iFluor647 conjugated

$670
M05077-carrier-free

Carrier Free

$370

More conjugates/formats

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M05077
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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