Product Info Summary
SKU: | P07769-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody
SKU/Catalog Number
P07769-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody (Catalog # P07769-1). Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07769-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1244 of the NR2C subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P07769-1 is reactive to GRIN2C in Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
52 kDa
Calculated molecular weight
134209 MW
Background of NMDAR2C
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser-1244 has been shown to regulate NMDA receptor channel function (Chen et al., 2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P07769-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor phosphorylated at Ser1244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For GRIN2C (Source: Uniprot.org, NCBI)
Gene Name
GRIN2C
Full Name
Glutamate receptor ionotropic, NMDA 2C
Weight
134209 MW
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
GluN2C antibody, Glutamate [NMDA] receptor subunit epsilon-3 antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody, Glutamate receptor ionotropic NMDA 2C antibody, Glutamate receptor ionotropic N methyl D aspartate 2C antibody, Grin2c antibody, Grin 2c antibody, GRIN2C antibody, GRIN 2C antibody, N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody, N Methyl D Aspartate Receptor Subtype 2C antibody, N methyl D aspartate receptor subunit 2C antibody, N-methyl D-aspartate receptor subtype 2C antibody, NMDAR2C antibody, NMDE3_HUMAN antibody, NR2C antibody, OTTHUMP00000041930 antibody GRIN2C GluN2C, NMDAR2C, NR2C glutamate ionotropic receptor NMDA type subunit 2C glutamate receptor ionotropic, NMDA 2C|N-methyl D-aspartate receptor subtype 2C|N-methyl-D-aspartate receptor subunit 2C|glutamate [NMDA] receptor subunit epsilon-3|glutamate receptor, ionotropic, N-methyl D-aspartate 2C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN2C, check out the GRIN2C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody (P07769-1)
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) Antibody
Question
Would anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 work on dog WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
Our lab technicians have not tested anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-30
Question
I see that the anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 works with WB, what is the protocol used to produce the result images on the product page?
N. Moore
Verified customer
Asked: 2019-12-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
See attached the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-11
Question
Would P07769-1 anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
You can see on the product datasheet, P07769-1 anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-14
Question
Is there a BSA free version of anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 available?
W. Krishna
Verified customer
Asked: 2019-01-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-NMDA Receptor NR2C Subunit (Ser1244) antibody P07769-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-11