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Product Info Summary
| SKU: | A03816 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Uba2/Sae2 Antibody
SKU/Catalog Number
A03816
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Uba2/Sae2 Antibody (Catalog # A03816). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Uba2/Sae2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03816)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human Uba2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03816 is reactive to UBA2 in Human
Reconstitution
Calculated molecular weight
71224 MW
Background of SAE2
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Mark H. L. Lambermon, Mol. Cell. Biol., Jun 2002; 22: 4346 - 4357.
Kyoung-Seok Ryu, J. Biol. Chem., Sep 2003; 278: 36621 - 36627.
R. Jrgen Dohmen, J. Biol. Chem., Jul 1995; 270: 18099.
Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03816 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, treated with UV (5mins), using Uba2 antibody A03816.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Uba2 antibody A03816.
Protein Target Info & Infographic
Gene/Protein Information For UBA2 (Source: Uniprot.org, NCBI)
Gene Name
UBA2
Full Name
SUMO-activating enzyme subunit 2
Weight
71224 MW
Superfamily
ubiquitin-activating E1 family
Alternative Names
SUMO-activating enzyme subunit 2;6.3.2.-;Anthracycline-associated resistance ARX;Ubiquitin-like 1-activating enzyme E1B;Ubiquitin-like modifier-activating enzyme 2;UBA2;SAE2, UBLE1B;HRIHFB2115; UBA2 ARX, HRIHFB2115, SAE2 ubiquitin like modifier activating enzyme 2 SUMO-activating enzyme subunit 2|SUMO-1 activating enzyme subunit 2|SUMO1 activating enzyme subunit 2|UBA2, ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance ARX|ubiquitin-like 1-activating enzyme E1B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBA2, check out the UBA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Uba2/Sae2 Antibody (A03816)
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No publications found for A03816
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