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Product Info Summary
| SKU: | A04545-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MED13 Antibody Picoband®
SKU/Catalog Number
A04545-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED13 Antibody Picoband® catalog # A04545-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04545-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MED13 recombinant protein (Position: L61-Y240).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04545-1 is reactive to MED13 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
300 kDa
Calculated molecular weight
34482 MW
Background of MED13
Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04545-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U2OS whole cell, human MCF-7 whole cell
ICC/IF: U20S cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MED13 using anti-MED13 antibody (A04545-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED13 antigen affinity purified polyclonal antibody (Catalog # A04545-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED13 at approximately 300KD. The expected band size for MED13 is at 239KD.
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Figure 2. IF analysis of MED13 using anti-MED13 antibody (A04545-1).
MED13 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MED13 Antibody (A04545-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A431 cells using anti-MED13 antibody (A04545-1).
Overlay histogram showing A431 cells stained with A04545-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED13 Antibody (A04545-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MED13 (Source: Uniprot.org, NCBI)
Gene Name
MED13
Full Name
Mediator of RNA polymerase II transcription subunit 13
Weight
34482 MW
Superfamily
Mediator complex subunit 13 family
Alternative Names
Mediator of RNA polymerase II transcription subunit 13; Activator-recruited cofactor 250 kDa component; ARC250; Mediator complex subunit 13; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; Trap240; Vitamin D3 receptor-interacting protein complex component DRIP250; DRIP250; MED13; ARC250, KIAA0593, THRAP1, TRAP240; MED13 ARC250, DRIP250, HSPC221, MRD61, THRAP1, TRAP240 mediator complex subunit 13 mediator of RNA polymerase II transcription subunit 13|activator-recruited cofactor 250 kDa component|mediator of RNA polymerase II transcription, subunit 13 homolog|thyroid hormone receptor-associated protein 1|thyroid hormone receptor-associated protein complex 240 kDa component|thyroid hormone receptor-associated protein complex component TRAP240|thyroid hormone receptor-associated protein, 240 kDa subunit|vitamin D3 receptor-interacting protein complex component DRIP250
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED13, check out the MED13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED13 Antibody Picoband® (A04545-1)
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1 Customer Q&As for Anti-MED13 Antibody Picoband®
Question
We are currently using anti-MED13 antibody A04545-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Z. Collins
Verified customer
Asked: 2013-11-14
Answer
The anti-MED13 antibody (A04545-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-14
