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Key=sku, value=A03816-2
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Key=created_at, value=2017-08-01 07:05:43
Key=updated_at, value=2024-10-25 05:01:59
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Key=meta_title, value=Anti-SAE2/UBA2 Antibody Picoband® | Bosterbio
Key=meta_description, value=Boster Bio Anti-SAE2/UBA2 Antibody Picoband® catalog # A03816-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=size, value=100 μg/vial
Key=protein_name, value=Heterogeneous nuclear ribonucleoprotein L
Key=gene_full_name, value=ubiquitin like modifier activating enzyme 2
Key=uniprot_id, value=Q9UBT2
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Key=predicted_reactivity
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Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=64133 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Dermatomyositis, Childhood Type:A06075-4|M03816-1^Pregnancy Complications, Infectious:M03816|M03816-1^Sexual Reproduction:PB9926
Key=description, value=Boster Bio Anti-SAE2/UBA2 Antibody Picoband® catalog # A03816-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-SAE2/UBA2 Antibody Picoband® catalog # A03816-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-SAE2/UBA2 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins
Key=gene_name, value=UBA2
Key=synonyms, value=SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115
Key=application_details, value=Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Key=subcellular_localization, value= Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.
Key=tissue_specificity, value=Duodenum, heart, lung and liver, but not thymus.
Key=protein_function, value= The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564).
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=90 kDa
Key=custom_attribute_2, value=WB: human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human A549 whole cell, human SK-OV-3 whole cell, human 22RV1 whole cell, human A431 whole cell, human COLO-320 whole cell
IHC: human colon cancer tissue, human mammary cancer tissue, rat testis tissue, mouse testis tissue
ICC/IF: A431 cell
FCM: A431 cell
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Anti-SAE2/UBA2 Antibody Picoband®

SAE2 antibody

Boster Bio Anti-SAE2/UBA2 Antibody Picoband® catalog # A03816-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03816-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-SAE2/UBA2 Antibody Picoband®

View all SAE2 Antibodies

SKU/Catalog Number

A03816-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SAE2/UBA2 Antibody Picoband® catalog # A03816-2. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SAE2/UBA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03816-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03816-2 is reactive to UBA2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

90 kDa

Calculated molecular weight

64133 MW

Background of SAE2

Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A03816-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human A549 whole cell, human SK-OV-3 whole cell, human 22RV1 whole cell, human A431 whole cell, human COLO-320 whole cell
IHC: human colon cancer tissue, human mammary cancer tissue, rat testis tissue, mouse testis tissue
ICC/IF: A431 cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For UBA2 (Source: Uniprot.org, NCBI)

Gene Name

UBA2

Full Name

SUMO-activating enzyme subunit 2

Weight

64133 MW

Superfamily

ubiquitin-activating E1 family

Alternative Names

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115 UBA2 ARX, HRIHFB2115, SAE2 ubiquitin like modifier activating enzyme 2 SUMO-activating enzyme subunit 2|SUMO-1 activating enzyme subunit 2|SUMO1 activating enzyme subunit 2|UBA2, ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance ARX|ubiquitin-like 1-activating enzyme E1B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on UBA2, check out the UBA2 Infographic

UBA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UBA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03816-2

100μg

$370
A03816-2-10ug

10μg sample (liquid)

$99
A03816-2-Biotin

100 μg Biotin conjugated

$570
A03816-2-Cy3

100 μg Cy3 conjugated

$570
A03816-2-Dylight488

100 μg Dylight488 conjugated

$570
A03816-2-Dylight550

100 μg Dylight550 conjugated

$570
A03816-2-Dylight594

100 μg Dylight594 conjugated

$570
A03816-2-FITC

100 μg FITC conjugated

$570
A03816-2-HRP

100 μg HRP conjugated

$570
A03816-2-APC

100 μg APC conjugated

$670
A03816-2-PE

100 μg PE conjugated

$670
A03816-2-iFluor647

100 μg iFluor647 conjugated

$670
A03816-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A03816-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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