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Product Info Summary
| SKU: | P00165 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00165
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody catalog # P00165. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00165)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IGO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-IRF3 (S386)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00165 is reactive to IRF3 in Human
Reconstitution
Observed Molecular Weight
120 kDa
Calculated molecular weight
47219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00165 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-IRF3 (S386) expression in MCF7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)
Gene Name
IRF3
Full Name
Interferon regulatory factor 3
Weight
47219 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 3;IRF-3;IRF3; IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF3, check out the IRF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody (P00165)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
Question
We have observed staining in human retina. What should we do? Is anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody supposed to stain retina positively?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to literature retina does express IRF3. According to Uniprot.org, IRF3 is expressed in small intestine peyer's patch, retina, spleen, eye kidney, erythroleukemia, liver, among other tissues. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-10
Question
We are currently using anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody P00165 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
S. Gonzalez
Verified customer
Asked: 2018-02-13
Answer
The anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody (P00165) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-13
Question
Our lab were satisfied with the WB result of your anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody. However we have seen positive staining in spleen cytoplasm using this antibody. Is that expected? Could you tell me where is IRF3 supposed to be expressed?
K. Huang
Verified customer
Asked: 2014-11-14
Answer
From literature, spleen does express IRF3. Generally IRF3 expresses in cytoplasm. Regarding which tissues have IRF3 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Eye, and Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Retina, Pubmed ID: 8524823
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-11-14
Question
We ordered your anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody for IF on small intestine peyer's patch in the past. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining small intestine peyer's patch as well as spleen in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Thomas
Verified customer
Asked: 2014-07-24
Answer
I viewed the website and datasheets of our anti-Phospho-IRF3 (S386) Rabbit Monoclonal antibody and it appears that P00165 has been validated on human in both IF and WB. Thus P00165 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-24
