Click image to see more details
Product Info Summary
| SKU: | PB9492 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CREB3L1 Antibody Picoband®
View all OASIS/CREB3L1 Antibodies
SKU/Catalog Number
PB9492
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CREB3L1 Antibody Picoband® catalog # PB9492. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CREB3L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9492)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CREB3L1 recombinant protein (Position: M1-M204). Human CREB3L1 shares 93.6% and 93.1% amino acid (aa) sequence identity with mouse and rat CREB3L1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9492 is reactive to CREB3L1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
57005 MW
Background of OASIS/CREB3L1
CREB3L1, known as Cyclic AMP-responsive element-binding protein 3-like protein 1, is mapped to 11p11.2. The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9492 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, HELA Whole Cell, A549 Whole Cell, MM231 Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CREB3L1 using anti-CREB3L1 antibody (PB9492).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug,
Lane 3: A549 Whole Cell Lysate at 40ug,
Lane 4: MM231 Whole Cell Lysate at 40ug,
Lane 5: COLO320 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L1 antigen affinity purified polyclonal antibody (Catalog # PB9492) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB3L1 at approximately 57 kDa. The expected band size for CREB3L1 is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CREB3L1 (Source: Uniprot.org, NCBI)
Gene Name
CREB3L1
Full Name
Cyclic AMP-responsive element-binding protein 3-like protein 1
Weight
57005 MW
Superfamily
bZIP family
Alternative Names
Cyclic AMP-responsive element-binding protein 3-like protein 1;cAMP-responsive element-binding protein 3-like protein 1;Old astrocyte specifically-induced substance;OASIS;Processed cyclic AMP-responsive element-binding protein 3-like protein 1;CREB3L1;OASIS;PSEC0238; Creb3l1|Oasis|cAMP responsive element binding protein 3-like 1|cyclic AMP-responsive element-binding protein 3-like protein 1|BBF-2 homolog|cAMP-responsive element-binding protein 3-like protein 1|old astrocyte specifically induced substance
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CREB3L1, check out the CREB3L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CREB3L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CREB3L1 Antibody Picoband® (PB9492)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CREB3L1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CREB3L1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-CREB3L1 Antibody Picoband®
Question
We are currently using anti-CREB3L1 antibody PB9492 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
The anti-CREB3L1 antibody (PB9492) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-25
