Anti-IRF3 Antibody (N-Term)

Product Name

Anti-IRF3 Antibody (N-Term)

View all IRF3 Antibodies

SKU/Catalog Number

M00165-5

Size

50 µl

Description

Boster Bio Anti-IRF3 Antibody (N-Term) (Catalog # M00165-5). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-IRF3 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00165-5)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This IRF3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from human IRF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00165-5 is reactive to IRF3 in Human

Reconstitution

Calculated molecular weight

47219 Da

Background of IRF3

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00165-5 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

Gene/Protein Information For IRF3 (Source: Uniprot.org, NCBI)

Alternative Names

Interferon regulatory factor 3, IRF-3, IRF3 IRF3 IIAE7 interferon regulatory factor 3 interferon regulatory factor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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