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Product Info Summary
| SKU: | PA2280 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Ras-related protein Rab-8A RAB8A Antibody Picoband®
SKU/Catalog Number
PA2280
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ras-related protein Rab-8A RAB8A Antibody catalog # PA2280. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras-related protein Rab-8A RAB8A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2280)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAB8A, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2280 is reactive to RAB8A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
23668 MW
Background of RAB8A
Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. It is mapped to 19p13.12. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. RAB8A regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of RAB8A into the cilium during assembly of this organelle. RAB8A has been shown to interact with Optineurin and MAP4K2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2280 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: Caco-2 whole cell, HepG2 whole cell,, A549 whole cell
IHC: Human Intestinal Cancer Tissue, Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-RAB8A antibody, PA2280, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-RAB8A antibody, PA2280, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Figure 3. Western blot analysis of RAB8A using anti-RAB8A antibody (PA2280).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: Caco-2 whole cell lysates,
lane 2: HepG2 whole cell lysates,
lane 3: A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB8A antigen affinity purified polyclonal antibody (Catalog # PA2280) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB8A at approximately 24KD. The expected band size for RAB8A is at 24KD.
Protein Target Info & Infographic
Gene/Protein Information For RAB8A (Source: Uniprot.org, NCBI)
Gene Name
RAB8A
Full Name
Ras-related protein Rab-8A
Weight
23668 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-8A;Oncogene c-mel;RAB8A;MEL, RAB8; RAB8A MEL, RAB8 RAB8A, member RAS oncogene family ras-related protein Rab-8A|mel transforming oncogene (RAB8 homolog)|mel transforming oncogene (derived from cell line NK14)|mel transforming oncogene (derived from cell line NK14)- RAB8 homolog|oncogene c-mel|ras-associated protein RAB8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB8A, check out the RAB8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ras-related protein Rab-8A RAB8A Antibody Picoband® (PA2280)
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Customer Q&As
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1 Customer Q&As for Anti-Ras-related protein Rab-8A RAB8A Antibody Picoband®
Question
We are currently using anti-RAB8A antibody PA2280 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
D. Mitchell
Verified customer
Asked: 2018-01-10
Answer
The anti-RAB8A antibody (PA2280) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-10
