Product Info Summary
SKU: | P02533 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal Antibody
SKU/Catalog Number
P02533
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal Antibody catalog # P02533. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02533)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FDE-3
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human Phospho-CrkII (Tyr221)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02533 is reactive to CRK in Human
Reconstitution
Observed Molecular Weight
85 kDa
Calculated molecular weight
33831 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02533 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
![p02533 wb p02533 wb](https://www.bosterbio.com/media/catalog/product/p/0/p02533-wb.jpg)
Click image to see more details
Western blot analysis of Phospho-CrkII (Tyr221) expression in (1) K562 cell lysate treated with AP; (2) K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CRK (Source: Uniprot.org, NCBI)
Gene Name
CRK
Full Name
Adapter molecule crk
Weight
33831 MW
Superfamily
CRK family
Alternative Names
adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; Crk; CRKII; p38; Proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian) CRK CRKII, p38 CRK proto-oncogene, adaptor protein adapter molecule crk|proto-oncogene c-Crk|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRK, check out the CRK Infographic
![CRK infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal Antibody (P02533)
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No publications found for P02533
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