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Product Info Summary
| SKU: | A00358 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Cdk6 Antibody Picoband®
SKU/Catalog Number
A00358
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cdk6 Antibody Picoband® catalog # A00358. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdk6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00358)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Cdk6 recombinant protein (Position: M1-A326).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00358 is reactive to CDK6 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
132494 MW
Background of CDK6
Cell division protein kinase 6, also called Plstire, is an enzyme that in humans is encoded by the CDK6 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. Radiation hybrid analysis and inclusion within a mapped clone place the CDK6 gene at 7q21. Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation and promotes G1/S transition. This gene also involved in initiation and maintenance of cell cycle exit during cell differentiation. It prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types. In addition, CDK6 plays a role in promoting the proliferation of beta-cells in pancreatic islets of Langerhans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00358 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, mouse RAW2647 whole cell
IHC: human intestinal cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.
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Figure 2. IHC analysis of Cdk6 using anti-Cdk6 antibody (A00358).
Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (A00358) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Cdk6 using anti-Cdk6 antibody (A00358).
Cdk6 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (A00358) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CDK6 (Source: Uniprot.org, NCBI)
Gene Name
CDK6
Full Name
Cyclin-dependent kinase 6
Weight
132494 MW
Superfamily
protein kinase superfamily
Alternative Names
Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; CDK6; CDKN6 CDK6 MCPH12, PLSTIRE cyclin dependent kinase 6 cyclin-dependent kinase 6|cell division protein kinase 6|serine/threonine-protein kinase PLSTIRE
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK6, check out the CDK6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdk6 Antibody Picoband® (A00358)
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Customer Q&As
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5 Customer Q&As for Anti-Cdk6 Antibody Picoband®
Question
We ordered your anti-Cdk6 antibody for WB on brain a few years ago. I am using mouse, and We want to use the antibody for IHC next. We want examining brain as well as tongue in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
I viewed the website and datasheets of our anti-Cdk6 antibody and I see that A00358 has been validated on mouse in both WB and IHC. Thus A00358 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-29
Question
I am interested in using your anti-Cdk6 antibody for oxidative stress induced senescence studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thanks for your inquiry. This A00358 anti-Cdk6 antibody is validated on human hela, hela whole cell lysates, k562 whole cell lysates, mouse sp20 whole cell lysates, intestinal cancer tissue, mammary cancer tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-31
Question
My colleagues were content with the WB result of your anti-Cdk6 antibody. However we have observed positive staining in tongue ruffle. using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?
M. Anderson
Verified customer
Asked: 2018-03-02
Answer
From literature, tongue does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-03-02
Question
We are currently using anti-Cdk6 antibody A00358 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
S. Collins
Verified customer
Asked: 2014-11-04
Answer
The anti-Cdk6 antibody (A00358) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-04
Question
We have observed staining in rat tongue. Are there any suggestions? Is anti-Cdk6 antibody supposed to stain tongue positively?
G. Zhao
Verified customer
Asked: 2014-01-22
Answer
According to literature tongue does express CDK6. According to Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-01-22
