Product Info Summary
SKU: | M05077-Dyl550 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)
View all AMPK beta 2/PRKAB2 Antibodies
SKU/Catalog Number
M05077-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody (monoclonal, 6G1) catalog # M05077-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05077-Dyl550)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
6G1
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M05077-Dyl550 is reactive to PRKAB2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
30.302kDa
Background of AMPK beta 2/PRKAB2
5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05077-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For PRKAB2 (Source: Uniprot.org, NCBI)
Gene Name
PRKAB2
Full Name
5'-AMP-activated protein kinase subunit beta-2
Weight
30.302kDa
Superfamily
5'-AMP-activated protein kinase beta subunit family
Alternative Names
5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2; PRKAB2 PRKAB2 protein kinase AMP-activated non-catalytic subunit beta 2 5-AMP-activated protein kinase subunit beta-2|5-AMP-activated protein kinase, beta-2 subunit|AMP-activated protein kinase beta 2 non-catalytic subunit|AMPK beta 2|AMPK beta-2 chain|AMPK subunit beta-2|protein kinase, AMP-activated, beta 2 non-catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAB2, check out the PRKAB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1) (M05077-Dyl550)
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Customer Q&As
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7 Customer Q&As for Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)
Question
Would M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
You can see on the product datasheet, M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-10
Question
Here is the WB image, lot number and protocol we used for liver using anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
Will anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 work for Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to the expression profile of liver, PRKAB2 is highly expressed in liver. So, it is likely that anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 will work for Flow Cytometry with liver.
Boster Scientific Support
Answered: 2019-12-25
Question
I have a question about product M05077-Dyl550, anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-20
Question
I see that the anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-30
Question
Do you have a BSA free version of anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 available?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-29