Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)

AMPK beta 2/PRKAB2 antibody

Boster Bio Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody (monoclonal, 6G1) catalog # M05077-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M05077-Dyl550
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)

View all AMPK beta 2/PRKAB2 Antibodies

SKU/Catalog Number

M05077-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody (monoclonal, 6G1) catalog # M05077-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05077-Dyl550)

Host

Mouse

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Monoclonal

Clone Number

6G1

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M05077-Dyl550 is reactive to PRKAB2 in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

30.302kDa

Background of AMPK beta 2/PRKAB2

5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05077-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For PRKAB2 (Source: Uniprot.org, NCBI)

Gene Name

PRKAB2

Full Name

5'-AMP-activated protein kinase subunit beta-2

Weight

30.302kDa

Superfamily

5'-AMP-activated protein kinase beta subunit family

Alternative Names

5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2; PRKAB2 PRKAB2 protein kinase AMP-activated non-catalytic subunit beta 2 5-AMP-activated protein kinase subunit beta-2|5-AMP-activated protein kinase, beta-2 subunit|AMP-activated protein kinase beta 2 non-catalytic subunit|AMPK beta 2|AMPK beta-2 chain|AMPK subunit beta-2|protein kinase, AMP-activated, beta 2 non-catalytic subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKAB2, check out the PRKAB2 Infographic

PRKAB2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKAB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Human AMPK beta 2 DyLight® 550 conjugated PRKAB2 Antibody(monoclonal, 6G1)

Question

Would M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

You can see on the product datasheet, M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-10

Question

Here is the WB image, lot number and protocol we used for liver using anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-08

Question

Will anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 work for Flow Cytometry with liver?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

According to the expression profile of liver, PRKAB2 is highly expressed in liver. So, it is likely that anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 will work for Flow Cytometry with liver.

Boster Scientific Support

Answered: 2019-12-25

Question

I have a question about product M05077-Dyl550, anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05077-Dyl550 anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-20

Question

I see that the anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-30

Question

Do you have a BSA free version of anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 available?

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human AMPK beta 2 DyLight® 550 conjugated antibody(monoclonal, 6G1) M05077-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-29

Order DetailsPrice
M05077-Dyl550

100μg

$515
M05077-Dyl550-carrier-free

Carrier Free

$515

Free Secondary Antibody

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M05077-Dyl550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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