Anti-PDL1 CD274 Monoclonal Antibody [4F2]

Product Name

Anti-PDL1 CD274 Monoclonal Antibody [4F2]

View all PD-L1 Antibodies

SKU/Catalog Number

M00109-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PDL1 CD274 Monoclonal Antibody [4F2] (Catalog # M00109-2). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

PD-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PDL1 CD274 Monoclonal Antibody [4F2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00109-2)

Host

Mouse

Contents

PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 4F2

Isotype

IgG1

Immunogen

PD-L1 antibody was raised against the extracellular domain of human PD-L1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.

Reactive Species

M00109-2 is reactive to CD274 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

33275 MW

Background of PD-L1

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00109-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CD274 (Source: Uniprot.org, NCBI)

Alternative Names

Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 |PDCD1 ligand 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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