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Product Info Summary
| SKU: | M02714 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PDE4A Mouse Monoclonal Antibody [Clone ID: OTI7B11]
View all Phosphodiesterase 4A/PDE4A Antibodies
SKU/Catalog Number
M02714
Size
100 µl
Description
Boster Bio Anti-PDE4A mouse monoclonal antibody, clone OTI7B11 (formerly 7B11). Catalog# M02714. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PDE4A Mouse Monoclonal Antibody [Clone ID: OTI7B11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02714)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI7B11
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02714 is reactive to PDE4A in Human, Rat
Reconstitution
Calculated molecular weight
98.143kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02714 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.
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Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-PDE4A monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either PDE4A (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE4A antibody (M02714)
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Flow cytometric Analysis of Jurkat cells
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Anti-PDE4A mouse monoclonal antibody (M02714) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A.
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Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (M02714).
Protein Target Info & Infographic
Gene/Protein Information For PDE4A (Source: Uniprot.org, NCBI)
Gene Name
PDE4A
Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
Weight
98.143kDa
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
cAMP-specific 3'-5'-cyclic phosphodiesterase 4A; DPDE2; DPDE2cAMP-specific phosphodiesterase; EC 3.1.4; EC 3.1.4.17; PDE4; PDE46; PDE4A; Phosphodiesterase 4A; phosphodiesterase 4A, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E2); phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase 4A, cAMP-specific; phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4 PDE4A DPDE2, PDE4, PDE46 phosphodiesterase 4A cAMP-specific 3,5-cyclic phosphodiesterase 4A|phosphodiesterase 4A, cAMP-specific (dunce|phosphodiesterase E2 dunce homolog, Drosophila|phosphodiesterase isozyme 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE4A, check out the PDE4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE4A Mouse Monoclonal Antibody [Clone ID: OTI7B11] (M02714)
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