Click image to see more details
Product Info Summary
| SKU: | PA2148 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Protein NDRG2 NDRG2 Antibody Picoband®
SKU/Catalog Number
PA2148
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein NDRG2 NDRG2 Antibody catalog # PA2148. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein NDRG2 NDRG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2148)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NDRG2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2148 is reactive to NDRG2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
40798 MW
Background of Ndrg2
Protein NDRG2, also known as KIAA1248, is a protein that in humans is encoded by the NDRG2 gene. This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. This gene is mapped to 14q11.2. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. It contributes to the regulation of the WNT signaling pathway. This gene may be involved in dendritic cell and neuron differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2148 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell, PANC Whole Cell
Validation Images & Assay Conditions
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Anti-NDRG2 antibody, PA2148, Western blotting
All lanes: Anti NDRG2 (PA2148) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Protein Target Info & Infographic
Gene/Protein Information For NDRG2 (Source: Uniprot.org, NCBI)
Gene Name
NDRG2
Full Name
Protein NDRG2
Weight
40798 MW
Superfamily
NDRG family
Alternative Names
Protein NDRG2;N-myc downstream-regulated gene 2 protein;Protein Syld709613;NDRG2;KIAA1248, SYLD; Ndrg2|AI182517, AU040374, Nd, Ndr2, SYLD|N-myc downstream regulated gene 2|protein NDRG2|N-myc downstream regulated 2|N-myc downstream-regulated gene 2 protein|protein Ndr2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDRG2, check out the NDRG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDRG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein NDRG2 NDRG2 Antibody Picoband® (PA2148)
Hello CJ!
PA2148 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sun Z, Shen L, Sun X, Tong G, Sun D, Han T, Yang G, Zhang J, Cao F, Yao L, Wang H. Histochem Cell Biol. 2011 Jan;135(1):27-35. Doi: 10.1007/S00418-010-0776-9. Epub 2010 Dec 31. Variation Of Ndrg2 And C-Myc Expression In Rat Heart During The Acute ...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Protein NDRG2 NDRG2 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for medial globus pallidus using anti-NDRG2 antibody PA2148. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for medial globus pallidus using anti-NDRG2 antibody PA2148. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
Does PA2148 anti-NDRG2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
It shows on the product datasheet, PA2148 anti-NDRG2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-07
Question
I see that the anti-NDRG2 antibody PA2148 works with WB, what is the protocol used to produce the result images on the product page?
K. Moore
Verified customer
Asked: 2019-01-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-25
