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Product Info Summary
| SKU: | M00085 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody
View all Proprotein Convertase 9/PCSK9 Antibodies
SKU/Catalog Number
M00085
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody catalog # M00085. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00085)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABFB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PCSK9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00085 is reactive to PCSK9 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
74286 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00085 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:20
Positive Control
WB: human A431 whole cell, human Hela whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (M00085).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified monoclonal antibody (Catalog # M00085) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 75 kDa. The expected band size for PCSK9 is at 75 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PCSK9 (Source: Uniprot.org, NCBI)
Gene Name
PCSK9
Full Name
Proprotein convertase subtilisin/kexin type 9
Weight
74286 MW
Superfamily
peptidase S8 family
Alternative Names
Proprotein convertase subtilisin/kexin type 9;3.4.21.-;Neural apoptosis-regulated convertase 1;NARC-1;Proprotein convertase 9;PC9;Subtilisin/kexin-like protease PC9;PCSK9;NARC1;PSEC0052; Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCSK9, check out the PCSK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCSK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody (M00085)
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Customer Q&As
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5 Customer Q&As for Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody
Question
Our team were happy with the WB result of your anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody. However we have seen positive staining in cerebellum teratocarcinoma cytoplasm. secreted. endosome. lysosome. using this antibody. Is that expected? Could you tell me where is PCSK9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
From literature, cerebellum teratocarcinoma does express PCSK9. Generally PCSK9 expresses in cytoplasm. secreted. endosome. lysosome. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2020-05-06
Question
We have seen staining in human cerebellum teratocarcinoma. Are there any suggestions? Is anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody supposed to stain cerebellum teratocarcinoma positively?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
From literature cerebellum teratocarcinoma does express PCSK9. From Uniprot.org, PCSK9 is expressed in right lobe of liver, cerebellum teratocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2019-10-03
Question
We are currently using anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody M00085 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody (M00085) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
We have tried in the past anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody for WB on cervix carcinoma in the past. I am using human, and We want to use the antibody for IP next. My question regards examining cervix carcinoma as well as right lobe of liver in our next experiment. Could give a recommendation on which antibody would work the best for IP?
T. Miller
Verified customer
Asked: 2018-12-17
Answer
I have checked the website and datasheets of our anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody and I see that M00085 has been tested on human in both WB and IP. Thus M00085 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-17
Question
We need using your anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody for post-translational protein modification studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
K. Johnson
Verified customer
Asked: 2017-12-21
Answer
We appreciate your inquiry. This M00085 anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for IP, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-21
