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Product Info Summary
| SKU: | PA2130 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PCSK9 Antibody Picoband®
View all Proprotein Convertase 9/PCSK9 Antibodies
SKU/Catalog Number
PA2130
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCSK9 Antibody catalog # PA2130. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCSK9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2130)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PCSK9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2130 is reactive to PCSK9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
66 kDa
Calculated molecular weight
74286 MW
Background of Proprotein Convertase 9/PCSK9
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. PCSK9 can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2130 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human A431 whole cell, human HepG2 whole cell, human MCF-7 whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PCSK9 using anti-PCSK9 antibody (PA2130).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # PA2130) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa. The expected band size for PCSK9 is at 74 kDa.
Click image to see more details
Figure 2. IHC analysis of PCSK9 using anti-PCSK9 antibody (PA2130).
PCSK9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCSK9 Antibody (PA2130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PCSK9 (Source: Uniprot.org, NCBI)
Gene Name
PCSK9
Full Name
Proprotein convertase subtilisin/kexin type 9
Weight
74286 MW
Superfamily
peptidase S8 family
Alternative Names
Proprotein convertase subtilisin/kexin type 9;3.4.21.-;Neural apoptosis-regulated convertase 1;NARC-1;Proprotein convertase 9;PC9;Subtilisin/kexin-like protease PC9;PCSK9;NARC1;PSEC0052; Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCSK9, check out the PCSK9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCSK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCSK9 Antibody Picoband® (PA2130)
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Customer Q&As
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17 Customer Q&As for Anti-PCSK9 Antibody Picoband®
Question
Is there a BSA free version of anti-PCSK9 antibody PA2130 available?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PCSK9 antibody PA2130 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-23
Question
We have observed staining in human cervix carcinoma erythroleukemia. Do you have any suggestions? Is anti-PCSK9 antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express PCSK9. From what I have seen in Uniprot.org, PCSK9 is expressed in right lobe of liver, cerebellum teratocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2019-10-24
Question
My lab would like using your anti-PCSK9 antibody for low-density lipoprotein particle clearance studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
S. Taylor
Verified customer
Asked: 2019-10-11
Answer
Thank you for your inquiry. This PA2130 anti-PCSK9 antibody is validated on a549 whole cell lysate, hela whole cell lysate, u87 whole cell lysate, panc whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PCSK9 antibody PA2130. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
I was wanting to use your anti-PCSK9 antibody for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
As indicated on the product datasheet, PA2130 anti-PCSK9 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
Would anti-PCSK9 antibody PA2130 work on dog WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Our lab technicians have not validated anti-PCSK9 antibody PA2130 on dog. You can run a BLAST between dog and the immunogen sequence of anti-PCSK9 antibody PA2130 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-04
Question
Is a blocking peptide available for product anti-PCSK9 antibody (PA2130)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We do provide the blocking peptide for product anti-PCSK9 antibody (PA2130). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-28
Question
Would anti-PCSK9 antibody PA2130 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
According to the expression profile of cervix carcinoma, PCSK9 is highly expressed in cervix carcinoma. So, it is likely that anti-PCSK9 antibody PA2130 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2019-04-17
Question
My question regarding product PA2130, anti-PCSK9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2130 anti-PCSK9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-01
Question
I see that the anti-PCSK9 antibody PA2130 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-26
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-PCSK9 antibody PA2130. Please let me know if you require anything else.
G. Edwards
Verified customer
Asked: 2018-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-15
Question
Does PA2130 anti-PCSK9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
You can see on the product datasheet, PA2130 anti-PCSK9 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-13
Question
My colleagues were well pleased with the WB result of your anti-PCSK9 antibody. However we have observed positive staining in cerebellum teratocarcinoma cytoplasm. secreted. endosome. lysosome. using this antibody. Is that expected? Could you tell me where is PCSK9 supposed to be expressed?
D. Dhar
Verified customer
Asked: 2017-03-20
Answer
From literature, cerebellum teratocarcinoma does express PCSK9. Generally PCSK9 expresses in cytoplasm. secreted. endosome. lysosome. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2017-03-20
Question
Is this PA2130 anti-PCSK9 antibody reactive to the isotypes of PCSK9?
S. Dhar
Verified customer
Asked: 2016-03-21
Answer
The immunogen of PA2130 anti-PCSK9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PCSK9(671-687aa AVTAVAICCRSRHLAQA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-21
Question
We ordered your anti-PCSK9 antibody for WB on cervix carcinoma in a previous experiment. I am using human, and I plan to use the antibody for IHC next. I am looking for examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
M. Edwards
Verified customer
Asked: 2014-12-02
Answer
I have checked the website and datasheets of our anti-PCSK9 antibody and it appears that PA2130 has been validated on human in both WB and IHC. Thus PA2130 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-12-02
Question
We need to test anti-PCSK9 antibody PA2130 on human cervix carcinoma for research purposes, then I may be interested in using anti-PCSK9 antibody PA2130 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2014-08-04
Answer
The products we sell, including anti-PCSK9 antibody PA2130, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-04
Question
We are currently using anti-PCSK9 antibody PA2130 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
V. Banerjee
Verified customer
Asked: 2014-02-10
Answer
The anti-PCSK9 antibody (PA2130) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-10
