Product Info Summary
SKU: | PB10042 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Apolipoprotein B/APOB Antibody Picoband®
SKU/Catalog Number
PB10042
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apolipoprotein B/APOB Antibody Picoband® catalog # PB10042. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein B/APOB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10042)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450). Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10042 is reactive to APOB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
515605 MW
Background of APOB
Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10042 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 2. IHC analysis of Apolipoprotein CIII using anti-Apolipoprotein CIII antibody (PB10042).
Apolipoprotein CIII was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein CIII Antibody (PB10042) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of Apolipoprotein CIII using anti-Apolipoprotein CIII antibody (PB10042).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein CIII antigen affinity purified polyclonal antibody (Catalog # PB10042) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein CIII at approximately 250 kDa. The expected band size for Apolipoprotein CIII is at 516 kDa.
Protein Target Info & Infographic
Gene/Protein Information For APOB (Source: Uniprot.org, NCBI)
Gene Name
APOB
Full Name
Apolipoprotein B-100
Weight
515605 MW
Alternative Names
Apolipoprotein B-100;Apo B-100;Apolipoprotein B-48;Apo B-48;APOB; APOB FCHL2, FLDB, LDLCQ4, apoB-100, apoB-48 apolipoprotein B apolipoprotein B-100|apolipoprotein B (including Ag(x) )|apolipoprotein B48
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOB, check out the APOB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apolipoprotein B/APOB Antibody Picoband® (PB10042)
Hello CJ!
PB10042 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang Y,Zhao C,Kong Y,Tan P,Liu S,Liu Y,Zeng F,Yuan Y,Zhao B,Wang J.Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes.J Steroid Biochem Mol Biol.2021 Apr 2:105893.doi:10.101 6/j.jsbmb.2021.105893.Epub ahead of print.PMID:33819629.
Species: Holstein calves
PB10042 usage in article: APP:WB, SAMPLE:HEPATOCYTES, DILUTION:1:1000
Kong Y, Zhao C, Huang Y, Liu Y, Liu S, Guo Y, Li M, Xu T, Zhao B, Wang J. Angiopoietin-like protein 4 promotes very-low-density lipoprotein assembly and secretion in bovine hepatocytes in vitro. IUBMB Life. 2020 Nov 17. doi: 10.1002/iub.2403. Epub ahead o
Species: Calf
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Customer Q&As
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4 Customer Q&As for Anti-Apolipoprotein B/APOB Antibody Picoband®
Question
We are currently using anti-Apolipoprotein B/APOB antibody PB10042 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-Apolipoprotein B/APOB antibody (PB10042) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
Will anti-Apolipoprotein B/APOB antibody PB10042 work on horse WB with small intestine?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Our lab technicians have not tested anti-Apolipoprotein B/APOB antibody PB10042 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Apolipoprotein B/APOB antibody PB10042 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse small intestine in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
I am interested in to test anti-Apolipoprotein B/APOB antibody PB10042 on human jejunal mucosa for research purposes, then I may be interested in using anti-Apolipoprotein B/APOB antibody PB10042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Zhao
Verified customer
Asked: 2018-12-12
Answer
The products we sell, including anti-Apolipoprotein B/APOB antibody PB10042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-12
Question
Would anti-Apolipoprotein B/APOB antibody PB10042 work for IHC with jejunal mucosa?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to the expression profile of jejunal mucosa, APOB is highly expressed in jejunal mucosa. So, it is likely that anti-Apolipoprotein B/APOB antibody PB10042 will work for IHC with jejunal mucosa.
Boster Scientific Support
Answered: 2018-10-26