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Product Info Summary
| SKU: | EK1542 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and urine. |
Product info
Product Name
Human LDLR ELISA Kit PicoKine®
SKU/Catalog Number
EK1542
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LDLR ELISA Kit PicoKine® (96 Tests). Quantitate Human LDLR in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LDLR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1542)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A22-R788
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1542 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1542 is reactive to LDLR in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and urine.
Application Guarantee
EK1542 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LDLR
The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1542-CAP | Anti-Human LDLR Pre-coated 96-well strip microplate | 1 |
| EK1542-ST | Human LDLR Standard | 2 vials, 10 ng/tube |
| EK1542-DA | Human LDLR Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.065 | 0.126 | 0.166 | 0.263 | 0.455 | 0.780 | 1.405 | 2.088 |
Data Example Images
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Human LDLR PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LDLR, Dilution ratio of 1:50, concentration in serum and plasma is 14492-122421 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LDLR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 99 | 718 | 1399 | 99 | 692 | 1264 |
| Standard deviation | 6.7%4 | 39.49 | 93.46 | 6.43 | 39.44 | 97.32 |
| CV (%) | 6% | 5.5% | 6.7% | 6.5% | 5.7% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LDLR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 99 | 98 | 114 | 103 | 103 | 6.34 | 6.1% |
| Sample 2 | 718 | 709 | 713 | 678 | 704 | 15.62 | 2.2% |
| Sample 3 | 1395 | 1493 | 1364 | 1372 | 1406 | 51.5 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For LDLR (Source: Uniprot.Org, NCBI)
Gene Name
LDLR
Full Name
Low-density lipoprotein receptor
Weight
95376 MW
Superfamily
LDLR family
Alternative Names
Low-density lipoprotein receptor;LDL receptor;LDLR; LDLR FH, FHC, FHCL1, LDLCQ2 low density lipoprotein receptor low-density lipoprotein receptor|LDL receptor|low-density lipoprotein receptor class A domain-containing protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LDLR, check out the LDLR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LDLR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LDLR ELISA Kit PicoKine® (EK1542)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human LDLR ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-19
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-02-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-03
Question
Q: how do I analyze ELISA data? I measured LDLR level in serum.
S. Bhasin
Verified customer
Asked: 2019-08-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-13
Question
Q: how can I thaw whole blood sample for LDLR ELISA after freezing?
J. Liu
Verified customer
Asked: 2018-09-22
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LDLR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-09-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring LDLR using the EK1542 Human LDLR Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-02-12
Question
Q: if the enzyme conjugated LDLR antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LDLR antigen?
C. Tiwari
Verified customer
Asked: 2017-09-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-09-27
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of LDLR in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
P. Garcia
Verified customer
Asked: 2016-07-15
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LDLR ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-07-15
Question
Q: Can LDLR ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
E. Walker
Verified customer
Asked: 2015-07-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2015-07-03
Question
Q: is it okay to use EDTA plasma as samples in Human LDLR Picokine® ELISA Kit (Catalog # EK1542)?
W. Wu
Verified customer
Asked: 2014-10-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of LDLR causing disruption of its protein structure. LDLR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the LDLR ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-10-16
