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Product Info Summary
| SKU: | EK1147 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine®
View all Proprotein Convertase 9/PCSK9 ELISA kits
SKU/Catalog Number
EK1147
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine® (96 Tests). Quantitate Human PCSK9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1147)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S153-Q692
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1147 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1147 is reactive to PCSK9 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1147 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Proprotein Convertase 9/PCSK9
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins (LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1147-CAP | Anti-Human PCSK9 Pre-coated 96-well strip microplate | 1 |
| EK1147-ST | Human PCSK9 Standard | 2 vials, 10 ng/tube |
| EK1147-DA | Human PCSK9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.013 | 0.062 | 0.102 | 0.169 | 0.356 | 0.743 | 1.327 | 2.021 |
Data Example Images
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Human PCSK9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Proprotein Convertase 9/PCSK9, Dilution ratio of 1:50, concentration in serum and plasma is 60-120 ng/ml..
Some articles we found to cite concentrations of Proprotein Convertase 9/PCSK9 in samples: 19571328 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 229 | 1991 | 5404 | 215 | 1927 | 5944 |
| Standard deviation | 16.03 | 125.43 | 243.18 | 17.63 | 132.96 | 285.31 |
| CV (%) | 7% | 6.9% | 4.5% | 8.2% | 6.9% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Proprotein Convertase 9/PCSK9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 229 | 229 | 219 | 191 | 217 | 15.55 | 7.1% |
| Sample 2 | 1991 | 1900 | 1655 | 1887 | 1858 | 123.99 | 6.6% |
| Sample 3 | 5404 | 4654 | 4674 | 4865 | 4899 | 302.83 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For PCSK9 (Source: Uniprot.Org, NCBI)
Gene Name
PCSK9
Full Name
Proprotein convertase subtilisin/kexin type 9
Weight
74286 MW
Superfamily
peptidase S8 family
Alternative Names
Proprotein convertase subtilisin/kexin type 9;3.4.21.-;Neural apoptosis-regulated convertase 1;NARC-1;Proprotein convertase 9;PC9;Subtilisin/kexin-like protease PC9;PCSK9;NARC1;PSEC0052; Pcsk9|AI415265, AI747682, FH3, HCHOLA3, Na, Narc1, PC9|proprotein convertase subtilisin/kexin type 9|proprotein convertase subtilisin/kexin type 9|NARC-1|convertase subtilisin|neural apoptosis regulated convertase 1|proprotein convertase 9|proprotein convertase PC9|subtilisin/kexin-like protease PC9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PCSK9, check out the PCSK9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PCSK9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine® (EK1147)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated PCSK9 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PCSK9 antigen?
L. Hill
Verified customer
Asked: 2020-11-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-28
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
W. Maharaj
Verified customer
Asked: 2020-09-12
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-09-12
Question
Q: What is the optimal O.D. value for PCSK9 ELISA kit? I performed your PCSK9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PCSK9 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-06-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PCSK9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of PCSK9 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-06-10
Question
Q: how do I analyze ELISA data? I have performed PCSK9 level in cell lysates.
R. Perez
Verified customer
Asked: 2020-04-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-13
Question
Can your human PCSK9 ELISA Kit detect distinguishably the difference of heterodimer and free fragment of human PCSK9? Keyword: samples, form, application
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Our human PCSK9 ELISA Kit (EK1147) detects both heterodimer and free fragment of human PCSK9. It cannot detect the difference between them.
Boster Scientific Support
Answered: 2020-02-21
Question
Does the Human PCSK9/Proprotein Convertase 9 PicoKine
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
Human PCSK9/Proprotein Convertase 9 PicoKine
Boster Scientific Support
Answered: 2020-02-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-05-01
Question
Q: Can PCSK9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Williams
Verified customer
Asked: 2018-07-21
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-07-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement PCSK9 using the EK1147 Human PCSK9 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-07-13
