Anti-PAX8 (Renal Cell Marker) Monoclonal Antibody

Product Name

Anti-PAX8 (Renal Cell Marker) Monoclonal Antibody

View all PAX8 Antibodies

SKU/Catalog Number

M00943

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-PAX8 (Renal Cell Marker) Monoclonal Antibody (Catalog # M00943). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-PAX8 (Renal Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00943)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PAX8/1492

Isotype

IgG2b, kappa

Immunogen

Recombinant fragment (around aa 60-261) of human PAX8 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00943 is reactive to PAX8 in Dog, Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

48218 MW

Background of PAX8

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00943 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PAX8 (Source: Uniprot.org, NCBI)

Alternative Names

Paired box protein Pax-8;PAX8; PAX8 paired box 8 paired box protein Pax-8|paired domain gene 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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