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Product Info Summary
| SKU: | A00943-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PAX8 Antibody Picoband®
SKU/Catalog Number
A00943-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAX8 Antibody Picoband® catalog # A00943-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAX8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00943-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PAX8, which shares 95% amino acid (aa) sequence identity with both mouse and rat PAX8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00943-1 is reactive to PAX8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
50408 MW
Background of PAX8
Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene. This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00943-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human SW579 cell, human SW579 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PAX8 using anti-PAX8 antibody (A00943-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW579 cell lysate,
Lane 2: human SW579 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX8 antigen affinity purified polyclonal antibody (Catalog # A00943-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX8 at approximately 48KD. The expected band size for PAX8 is at 48KD.
Protein Target Info & Infographic
Gene/Protein Information For PAX8 (Source: Uniprot.org, NCBI)
Gene Name
PAX8
Full Name
Paired box protein Pax-8
Weight
50408 MW
Alternative Names
Paired box protein Pax-8; PAX8 PAX8 paired box 8 paired box protein Pax-8|paired domain gene 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAX8, check out the PAX8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAX8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAX8 Antibody Picoband® (A00943-1)
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Customer Q&As
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3 Customer Q&As for Anti-PAX8 Antibody Picoband®
Question
We are currently using anti-PAX8 antibody A00943-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-PAX8 antibody (A00943-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
We were content with the WB result of your anti-PAX8 antibody. However we have observed positive staining in cervix nucleus. using this antibody. Is that expected? Could you tell me where is PAX8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Based on literature, cervix does express PAX8. Generally PAX8 expresses in nucleus. Regarding which tissues have PAX8 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 14702039
Kidney, Pubmed ID: 1337742, 8413205, 15489334
Boster Scientific Support
Answered: 2020-01-30
Question
We have seen staining in rat cervix. What should we do? Is anti-PAX8 antibody supposed to stain cervix positively?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
According to literature cervix does express PAX8. According to Uniprot.org, PAX8 is expressed in right lobe of thyroid gland, kidney, cervix, among other tissues. Regarding which tissues have PAX8 expression, here are a few articles citing expression in various tissues:
Cervix, Pubmed ID: 14702039
Kidney, Pubmed ID: 1337742, 8413205, 15489334
Boster Scientific Support
Answered: 2018-01-03
