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Product Info Summary
| SKU: | A11557 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Dapper homolog 3 Dact3 Antibody
View all DACT3/Dapper 3 Antibodies
SKU/Catalog Number
A11557
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Dapper homolog 3 Dact3 Antibody (Catalog # A11557). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Dact3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Dapper homolog 3 Dact3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11557)
Host
Rabbit
Contents
Dact3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Dact3 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Dact3. The immunogen is located within amino acids 370 - 420 of Dact3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of Coronin 7 are known to exist
Reactive Species
A11557 is reactive to DACT3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
64949 MW
Background of DACT3/Dapper 3
The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact3, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact3 is expressed in the ventral region of maturing somites, limb bud and branchial arch mesenchyme, embryonic CNS, and the adult brain. Recent evidence shows that Dact3 acts as a negative regulator Wnt/beta-catenin signaling that is repressed at the transcriptional level in colorectal cancer and this repression is associated with bivalent histone modifications. This repression can be reversed by pharmacological agents that targets both histone methylation and deacetylation, suggesting that Dact3 may be a potential target for therapeutic treatment of this cancer. At least three isoforms of Dact3 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11557 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Dact3 antibody can be used for detection of Dact3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Dact3 in rat brain tissue lysate with Dact3 antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DACT3 (Source: Uniprot.org, NCBI)
Gene Name
DACT3
Full Name
Dapper homolog 3
Weight
64949 MW
Superfamily
dapper family
Alternative Names
RRR1, DAPPER3, RRR1, Dapper homolog 3, Antagonist of beta-catenin Dapper homolog 3 DACT3 DAPPER3, RRR1 dishevelled binding antagonist of beta catenin 3 dapper homolog 3|antagonist of beta-catenin Dapper homolog 3|arginine-rich region 1 protein|dapper, antagonist of beta-catenin, homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DACT3, check out the DACT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DACT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dapper homolog 3 Dact3 Antibody (A11557)
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