Anti-PARP2 Antibody Picoband™

Product Name

Anti-PARP2 Antibody Picoband™

View all PARP2 Antibodies

SKU/Catalog Number

A03270-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PARP2 Antibody Picoband™ catalog # A03270-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PARP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03270-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PARP2 recombinant protein (Position: R13-E253).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03270-2 is reactive to PARP2 in Human, Rat

Applications

A03270-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

66 kDa

Calculated molecular weight

Background of PARP2

Poly [ADP-ribose] polymerase 2 is an enzyme that in humans is encoded by the PARP2 gene. This gene encodes poly (ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PARP2 using anti-PARP2 antibody (A03270-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP2 antigen affinity purified polyclonal antibody (Catalog # A03270-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP2 at approximately 66KD. The expected band size for PARP2 is at 66KD.

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Figure 2. IF analysis of PARP2 using anti-PARP2 antibody (A03270-2).
PARP2 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PARP2 Antibody (A03270-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-PARP2 antibody (A03270-2).
Overlay histogram showing HL-60 cells stained with A03270-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP2 Antibody (A03270-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For PARP2 (Source: Uniprot.org, NCBI)

Alternative Names

ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2; ADPRT-2; ADPRT2pADPRT-2; ADPRTL2EC 2.4.2.30; ADPRTL3; hPARP-2; NAD(+) ADP-ribosyltransferase 2; PARP-2; poly (ADP-ribose) polymerase 2; poly (ADP-ribose) polymerase family, member 2; poly (ADP-ribosyl) transferase-like 2; poly [ADP-ribose] polymerase 2; poly(ADP-ribose) synthetase; Poly[ADP-ribose] synthase 2; poly[ADP-ribose] synthetase 2 PARP2 ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2, pADPRT-2 poly(ADP-ribose) polymerase 2 poly [ADP-ribose] polymerase 2|ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2|ADP-ribosyltransferase diphtheria toxin-like 2|ADPRT-2|DNA ADP-ribosyltransferase PARP2|NAD(+) ADP-ribosyltransferase 2|hPARP-2|poly (ADP-ribose) polymerase family, member 2|poly (ADP-ribosyl) transferase-like 2|poly[ADP-ribose] synthase 2|poly[ADP-ribose] synthetase 2|protein poly-ADP-ribosyltransferase PARP2

For more info on PARP2, check out the PARP2 Infographic

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In this infographic, you will see the following information for PARP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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