Anti-human CD117 Monoclonal Antibody FITC Conjugated, Flow Validated
|Size||25 Tests, 100 Tests, 200 Tests|
|Product Name||Anti-human CD117 Monoclonal Antibody FITC Conjugated, Flow Validated|
|Storage & Handling||4°C and protect from prolonged exposure to light. Do not freeze.|
|Size||25 Tests, 100 Tests, 200 Tests|
|Description||Mouse Monoclonal Anti-human CD117 FITC Conjugated, designed for Flow Cytometry and validated by Flow Cytometry using Human cells.|
|Cite This Product||Anti-human CD117 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01335-FITC)|
|Contents/Buffer||PBS, pH7.2, 0.09% NaN3 and 0.2% (w/v) BSA|
|Immunogen||Human erythroleukemia cell line OCIM1|
Assay Dilutions Overview
User need to optimize the dilution ratio for this antibody
Images And Assay Conditions
K562 cells stained with FITC
mouse anti-human CD117 (clone BA7.3C.9) (red histogram). K562 cells stained with FITC conjugated mouse IgG2a isotype control (Blue histogram).
Protein Target Info (Source: Uniprot.org)
|Protein Name||Mast/stem cell growth factor receptor Kit|
|Tissue Specificity||Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .|
|Alternative Names||Mast/stem cell growth factor receptor Kit;SCFR;220.127.116.11;Piebald trait protein;PBT;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;CD117;KIT;SCFR;|
|Subcellular Localization||Isoform 1: Cell membrane; Single-pass type I membrane protein.|
|Molecular Weight||109865 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. .|
*You can search these to find other products in these research areas.
|Background||SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.|
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