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Product Info Summary
| SKU: | A03270-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PARP-2 Antibody
SKU/Catalog Number
A03270-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PARP-2 Antibody catalog # A03270-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARP-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03270-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PARP2. AA range:151-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03270-1 is reactive to PARP2 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
66206 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03270-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using PARP-2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PARP2 (Source: Uniprot.org, NCBI)
Gene Name
PARP2
Full Name
Poly [ADP-ribose] polymerase 2
Weight
66206 MW
Alternative Names
Poly [ADP-ribose] polymerase 2;PARP-2;hPARP-2;2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 2;ARTD2;NAD (+) ADP-ribosyltransferase 2;ADPRT-2;Poly[ADP-ribose] synthase 2;pADPRT-2;PARP2;ADPRT2, ADPRTL2; PARP2 ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2, pADPRT-2 poly(ADP-ribose) polymerase 2 poly [ADP-ribose] polymerase 2|ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2|ADP-ribosyltransferase diphtheria toxin-like 2|ADPRT-2|DNA ADP-ribosyltransferase PARP2|NAD(+) ADP-ribosyltransferase 2|hPARP-2|poly (ADP-ribose) polymerase family, member 2|poly (ADP-ribosyl) transferase-like 2|poly[ADP-ribose] synthase 2|poly[ADP-ribose] synthetase 2|protein poly-ADP-ribosyltransferase PARP2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARP2, check out the PARP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PARP-2 Antibody (A03270-1)
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Customer Q&As
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4 Customer Q&As for Anti-PARP-2 Antibody
Question
We are currently using anti-PARP-2 antibody A03270-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The anti-PARP-2 antibody (A03270-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-04
Question
Can you help my question with product A03270-1, anti-PARP-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Evans
Verified customer
Asked: 2019-08-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03270-1 anti-PARP-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-20
Question
Would anti-PARP-2 antibody A03270-1 work on canine WB with fetal brain?
J. Krishna
Verified customer
Asked: 2019-02-15
Answer
Our lab technicians have not tested anti-PARP-2 antibody A03270-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PARP-2 antibody A03270-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-15
Question
I was wanting to use your anti-PARP-2 antibody for WB for mouse fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse fetal brain identification?
G. Li
Verified customer
Asked: 2016-12-22
Answer
As indicated on the product datasheet, A03270-1 anti-PARP-2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-22
