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Product Info Summary
| SKU: | PB9258 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-c-Kit Antibody Picoband®
View all CD117/c-kit Antibodies
SKU/Catalog Number
PB9258
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-c-Kit Antibody Picoband® catalog # PB9258. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Kit Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9258)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9258 is reactive to KIT in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
109 kDa
Calculated molecular weight
109865 MW
Background of CD117/c-kit
SCFR (Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9258 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106cells
Positive Control
WB: Recombinan Human C-Kit Protein 05ng,, HEPG2 Whole Cell,
IHC: human intestinal cancer tissue
FCM: K562 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of C-Kit using anti-C-Kit antibody (PB9258).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinan Human C-Kit Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # PB9258) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Kit at approximately
49KD. The expected band size for C-Kit is at 49KD.
Click image to see more details
Figure 2. Western blot analysis of C-Kit using anti-C-Kit antibody (PB9258).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPG2 Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # PB9258) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Kit at approximately
109KD. The expected band size for C-Kit is at 109KD.
Click image to see more details
Figure 3. IHC analysis of C-Kit using anti-C-Kit antibody (PB9258).
C-Kit was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C-Kit Antibody (PB9258) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of K562 cells using anti-C-Kit antibody (PB9258).
Overlay histogram showing K562 cells stained with PB9258 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (PB9258,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KIT (Source: Uniprot.org, NCBI)
Gene Name
KIT
Full Name
Mast/stem cell growth factor receptor Kit
Weight
109865 MW
Superfamily
protein kinase superfamily
Alternative Names
Mast/stem cell growth factor receptor Kit;SCFR;2.7.10.1;Piebald trait protein;PBT;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;CD117;KIT;SCFR; KIT C-Kit, CD117, MASTC, PBT, SCFR KIT proto-oncogene, receptor tyrosine kinase mast/stem cell growth factor receptor Kit|c-Kit protooncogene|p145 c-kit|piebald trait protein|proto-oncogene c-Kit|proto-oncogene tyrosine-protein kinase Kit|soluble KIT variant 1|tyrosine-protein kinase Kit|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIT, check out the KIT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Kit Antibody Picoband® (PB9258)
Hello CJ!
PB9258 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Number of Interstitial Cells of Cajal Differs Among Different Subtypes of Achalasia and is Related to Patients Prognosis
Isolation, Culture and Characterization of Cardiac Progenitor Cells Derived from Human Embryonic Heart Tubes
Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis
Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.
Yang B, Zhou Xc, Lan C. Mol Med Rep. 2015 Apr;11(4):2735-40. Doi: 10.3892/Mmr.2014.3039. Epub 2014 Dec 3. Impact Of The Alterations In The Interstitial Cells Of Cajal On Intestinal Motility In Post-Infection Irritable Bowel Syndrome.
Ge C, Zhang C, Ye J, Tang X, Wu Y. Cell Biol Int. 2007 Oct;31(10):1251-6. Epub 2007 May 13. Ginsenosides Promote Proliferation Of Chicken Primordial Germ Cells Via Pkc-Involved Activation Of Nf-Kappab.
Bin Wt, Ma Lm, Xu Q, Shi Xl. World J Gastroenterol. 2012 Jan 28;18(4):309-22. Doi: 10.3748/Wjg.V18.I4.309. Embryonic Hepatocyte Transplantation For Hepatic Cirrhosis: Efficacy And Mechanism Of Action.
Liu W, Peng Qx, Lin Xl, Luo Ch, Jiang Mj, Mo Zx, Yung Kk. Fitoterapia. 2014 Jan;92:16-22. Doi: 10.1016/J.Fitote.2013.10.002. Epub 2013 Oct 17. Effect Of Rhynchophylline On The Expression Of P-Creb And Sc-Fos In Triatum And Hippocampal Ca1 Area Of ...
Zhang H, Li L, Xu Gy, Mei Yw, Zhang Jj, Murong Sx, Sun Sg, Tong Et. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):354-8. Doi: 10.1007/S11596-014-1282-4. Epub 2014 Jun 18. Changes Of C-Fos, Malondialdehyde And Lactate In Brain Tissue After ...
Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...
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Customer Q&As
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1 Customer Q&As for Anti-c-Kit Antibody Picoband®
Question
We are currently using anti-c-Kit antibody PB9258 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
C. Anderson
Verified customer
Asked: 2016-05-24
Answer
The anti-c-Kit antibody (PB9258) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-24
