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Product Info Summary
| SKU: | PA1729 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PAK6 Antibody Picoband®
SKU/Catalog Number
PA1729
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PAK6 Antibody catalog # PA1729. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1729)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1729 is reactive to PAK6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
74869 MW
Background of PAK6
Serine/threonine-protein kinase PAK 6 (p21 Protein-activated Kinase 6) is an enzyme that in humans is encoded by the PAK6 gene. The PAK6 gene is mapped to chromosome 15q15. based on an alignment of the PAK6 sequence with the genomic sequence. This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1729 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: HELA Whole Cell, 293T Whole Cell, RAJI Whole Cell, COLO320 Whole Cell
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-PAK6 antibody, PA1729, Western blotting
All lanes: Anti PAK6 (PA1729) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
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Anti-PAK6 antibody, PA1729, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For PAK6 (Source: Uniprot.org, NCBI)
Gene Name
PAK6
Full Name
Serine/threonine-protein kinase PAK 6
Weight
74869 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase PAK 6;2.7.11.1;PAK-5;p21-activated kinase 6;PAK-6;PAK6;PAK5; PAK6 PAK5 p21 (RAC1) activated kinase 6 serine/threonine-protein kinase PAK 6|p21 protein (Cdc42/Rac)-activated kinase 6|p21(CDKN1A)-activated kinase 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK6, check out the PAK6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAK6 Antibody Picoband® (PA1729)
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No publications found for PA1729
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PAK6 Antibody Picoband®
Question
We are currently using anti-PAK6 antibody PA1729 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-PAK6 antibody (PA1729) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
Will anti-PAK6 antibody PA1729 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not tested anti-PAK6 antibody PA1729 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PAK6 antibody PA1729 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
Is there a BSA free version of anti-PAK6 antibody PA1729 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAK6 antibody PA1729 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
Would anti-PAK6 antibody PA1729 work for WB with prefrontal cortex?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
According to the expression profile of prefrontal cortex, PAK6 is highly expressed in prefrontal cortex. So, it is likely that anti-PAK6 antibody PA1729 will work for WB with prefrontal cortex.
Boster Scientific Support
Answered: 2019-12-19
Question
I have a question about product PA1729, anti-PAK6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1729 anti-PAK6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-05
Question
See below the WB image, lot number and protocol we used for prefrontal cortex using anti-PAK6 antibody PA1729. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-23
Question
Is this PA1729 anti-PAK6 antibody reactive to the isotypes of PAK6?
S. Patel
Verified customer
Asked: 2015-11-11
Answer
The immunogen of PA1729 anti-PAK6 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human PAK6(16-32aa QNFQHRVHTSFDPKEGK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-11
